17-6003-73
            
IMM. DRYSTRIP PH 3-11 NL, 7 CM
        
        

            
17-6003-74
            
IMM. DRYSTRIP PH 3-11 NL, 11CM
        
        

            
17-6003-75
            
IMM.DRYSTRIP PH 3-11 NL, 13CM
        
        

            
17-6003-76
            
IMM. DRYSTRIP PH 3-11 NL, 18CM
        
        

            
17-6003-77
            
IMM. DRYSTRIP PH 3-11 NL, 24CM
        
    


Immobiline™ DryStrip Gels
Technical Information


 

 

Immobiline™ DryStrip pH 4-7 run according to standard procedures.

A) Spots are elongated in the second dimension due to skewed bands in the first dimension.

B) Improved Immobiline™ DryStrip gels provide a more stable pH gradient and better spot definition in the second dimension.
 
Immobiline™ DryStrip gels (IPG strips) are used for isoelectric focusing (IEF), run as the first dimension of 2-D electrophoresis or as a separate application. IPG strips contain a preformed pH gradient immobilized in precast polyacrylamide gels on a plastic backing. A comprehensive range of overlapping IPG strips covering narrow, medium, and wide pH ranges are available in various strip lengths, and the strips are individually packaged and bar coded for convenient handling and sample tracking.
 
Immobiline™ DryStrip pH 3-11 NL covers most proteins found in prokaryotic and eukaryotic cells, while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear (NL) to distribute the proteins evenly over the gel length and obtain maximal resolution.
 
Immobiline™ DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of protein. Samples can also be loaded after rehydration. The first dimension separation is carried out using Ettan™ IPGphor™ 3 IEF system or Multiphor™ II flatbed system. When used for 2-D DIGE applications, the gels give accurate and reliable 2-D results.
 


    

        

            
TECHNICAL SPECIFICATIONS
        
        

            
 
            
 
        
        

            
Gel dimensions 
            
70 × 3 × 0.5 mm, 110 × 3 × 0.5 mm,

            130 × 3 × 0.5 mm, 180 × 3 × 0.5 mm,

            240 × 3 × 0.5 mm 
        
        

            
Gel matrix 
            
Polyacrylamide T = 4%, C = 3% 
        
        

            
Gel backing 
            
Polyester film 
        
        

            
Storage 
            
-20° C 
        
        

            
Shelf life 
            
18 months 
        
    


 
SELECTION GUIDE - Immobiline™ DryStrip Gels and IPG Buffers
 
Guidelines for choosing Immobiline DryStrip gels 

 

    
Use shorter strips for fast screening or when only the most abundant proteins are of interest.

     
    
Use longer strips for maximal resolution and loading capacity.

     
    
To overview total protein distribution, use pH 3-11 NL strips.

     
    
For increased resolution between pH 5-7, use a nonlinear gradient pH 3-10 strip to distribute the proteins more evenly over the strip.

     
    
Combine pH 3-7 NL and pH 6-11, pH 3-7 NL and pH 6-9, or select from 3-5.6 NL, 5.3-6.5, 6.2-7.5, and 7-11 NL when more detail is required.

     
    
Use narrow pH range strips (1 pH unit) to closely study proteins in the regions of interest.

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