USER^® 酶

在售热敏 USER II 酶（NEB #M5508）和热稳定 USER III 酶（NEB #M5509）。

我们很高兴地宣布，所有反应缓冲液均已不含 BSA（牛血清白蛋白）。从 2021 年 4 月开始，NEB 将含有 BSA 的反应缓冲液转换为含有重组白蛋白（rAlbumin）的缓冲液，用于限制性内切酶和某些 DNA 修饰酶。如需了解更多信息，请访问 www.neb.cn/BSA-free。

USER (Uracil-Specific Excision Reagent) Enzyme (1) generates a single nucleotide gap at the location of a uracil residue.

Applications of USER include:

Directional RNA Seq
NEBNext adaptor cleavage
USER cloning

Figure 1: USER Enzyme

Uracil DNA glycosylase (UDG) catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3´ and 5´ sides of the abasic site so that base-free deoxyribose is released. USER (Uracil-Specific Excision Reagent) Enzyme, combines these two enzymatic activities to generate a single nucleotide gap at the location of a uracil residue.