预优化DNA转染试剂-LNCaP-克隆与表达-试剂-生物在线
预优化DNA转染试剂-LNCaP

预优化DNA转染试剂-LNCaP

商家询价

产品名称: 预优化DNA转染试剂-LNCaP

英文名称: GenJet™ In Vitro DNA Tranfection Reagent for LNCaP Cells

产品编号: SL100489-LNCaP

产品价格: 0

产品产地: 美国

品牌商标: SignaGen

更新时间: null

使用范围: null

济南思科生物科技有限公司
  • 联系人 :
  • 地址 : 济南市经六路689号绿地·玫瑰坊A座734号
  • 邮编 : 250021
  • 所在区域 : 山东
  • 电话 : 159****9896
  • 传真 : 0531-85912162
  • 邮箱 : jnqsw@163.com; info@signagen-china.com

Description

GenJet™ DNA In Vitro Tranfection Reagent for LNCap is pre-optimized for transfecting LNCap cells. LNCap cells are a cell line of human cells commonly used in the field of oncology. LNCaP cells are androgen-sensitive human prostate adenocarcinoma cells derived from the left supraclavicular lymph node metastasis from a 50-year-old caucasian male in 1977. They are adherent epithelial cells growing in aggregates and as single cells.

Refer to the following optimal transfection conditions for maximal transfection efficiency on LNCap cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


95%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
No
48 hours


EGFP
pEGFP-N3 (CMV promoter) 

80%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


GenJet™ reagent is optimized for LNCap cells. LNCap cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™ (Ver. II). The efficiency was checked 48 hours post transfection 

Data Sheet .pdf