T细胞和B细胞活性miRNA PCR芯片(T-Cell & B-Cell Activation miScript miRNA PCR Array(T-Cell
产品名称： T细胞和B细胞活性miRNA PCR芯片(T-Cell & B-Cell Activation miScript miRNA PCR Array(T-Cell
英文名称： T-Cell & B-Cell Activation miScript miRNA PCR Array
产品编号： yb239
产品价格： 4800
产品产地： null
品牌商标： null
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 上海市闵行区浦江镇联航路1239弄8B号3楼
- 邮编 : 201112
- 所在区域 : 上海
- 电话 : 181****9791
- 传真 : 021-34786156
- 邮箱 : gulingzhi@yingbio.com
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miScript miRNA PCR Array |
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T细胞和B细胞活化miRNA 芯片用于研究参与T细胞和B细胞的活化和分化的84 miRNA的表达。这个芯片为科研人员提供了一个方便快速的方法来分析特定阶段T细胞和B细胞发育最相关的miRNA。造血干细胞分化成淋巴和髓系祖细胞。淋巴祖细胞迁移到胸腺, 保证T细胞谱系从双阴性(CD4-/CD8-)阶段开始，发展成T细胞。通过双阳性细胞(CD4 + / CD8 +)阶段,以单一阳性阶段结束,生成未分化T细胞CD4 +或CD8 +。在适应性免疫反应过程, CD8 + T细胞与抗原相互作用和激活,分化成效应T细胞和潜在的记忆T细胞,而CD4 + T细胞被抗原激活分化成辅助T细胞。B细胞在脾中成熟并保持未分化状态,直到它们遇到抗原。激活B细胞分化成血浆B细胞或记忆B细胞,或经过一个中间分化的步骤和转换抗体类型成为生发中心B细胞。T细胞和B细胞的激活涉及一序列的步骤和仔细的校时信号通路。特定的miRNA在微调重要免疫细胞的分化和激活中扮演重要角色,B细胞或T细胞功能的失调可以导致免疫缺陷或癌症。张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地检测参与T细胞和B细胞的激活的miRNA的表达。
miRNA PCR芯片用于分子生物学应用。本产品不用于疾病的诊断、预防和治疗。
T-Cell Differentiation*:
Double Negative (CD4-/CD8-):let-7d-5p, let-7e-5p, miR-126-3p, miR-128, miR-146b-5p, miR-15a-5p, miR-17-5p, miR-17-3p, miR-181c-5p, miR-191-5p, miR-19a-3p, miR-199a-5p, miR-20a-5p, miR-20b-5p, miR-221-3p, miR-222-3p, miR-223-3p, miR-28-5p, miR-29c-3p, miR-30e-5p, miR-342-3p, miR-423-5p, miR-93-5p, miR-98-5p.
Double Positive (CD4+/CD8+):let-7b-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-19b-3p.
CD4+ Naïve:miR-132-3p, miR-146a-5p, miR-182-5p, miR-184, miR-25-3p, miR-326, miR-92a-3p.
CD8+ Naïve:let-7a-5p, let-7c, let-7f-5p, let-7g-5p, miR-130b-3p, miR-139-5p, miR-142-3p, miR-150-5p, miR-155-5p, miR-15a-3p, miR-16-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-30b-5p, miR-30c-5p, miR-30d-5p.
CD8+ Effector:miR-147a, miR-148a-3p, miR-18a-5p, miR-27a-3p, miR-27b-3p.
CD8+ Memory:let-7i-5p, miR-106b-5p, miR-142-5p, miR-15b-5p, miR-17-5p, miR-21-5p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-29a-3p, miR-31-5p.
B-Cell Differentiation*:
Naïve:let-7a-5p, let-7b-5p, let-7d-5p, let-7g-5p, let-7i-5p, miR-101-3p, miR-132-3p, miR-142-3p, miR-142-5p, miR-150-5p, miR-181c-5p, miR-195-5p, miR-204-5p, miR-214-3p, miR-221-3p, miR-222-3p, miR-223-3p, miR-29b-3p, miR-30e-5p, miR-331-3p, miR-92a-3p.
Germinal Center:miR-106b-5p, miR-130b-3p, miR-132-3p, miR-148a-3p, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-17-3p, miR-181a-5p, miR-181b-5p, miR-191-5p, miR-19a-3p, miR-19b-3p, miR-210, miR-23b-3p, miR-25-3p, miR-28-5p, miR-30d-5p, miR-93-5p, miR-98-5p.
Memory:miR-100-5p, miR-125b-5p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29c-3p, miR-30b-5p, miR-30c-5p, miR-34a-5p.
Differentially Expressed in Tregs:miR-100-5p, miR-125b-5p, miR-146a-5p, miR-181c-5p, miR-20b-5p, miR-21-5p, miR-31-5p, miR-335-5p, miR-365a-3p, miR-99a-5p.
T-Cell Activation:let-7e-5p, let-7g-5p, let-7i-5p, miR-106b-5p, miR-139-5p, miR-142-5p, miR-146a-5p, miR-146b-5p, miR-155-5p, miR-15a-5p, miR-15a-3p, miR-181a-5p, miR-181c-5p, miR-195-5p, miR-20b-5p, miR-214-3p, miR-223-3p, miR-23a-3p, miR-23b-3p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-342-3p, miR-346, miR-574-3p, miR-98-5p.