The Zyppy™ Plasmid Miniprep Kit features a Pellet-Free™ modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, then purify using our Fast-Spin™ column technology (alternatively, the samples may be processed by the classical centrifugation method). Additionally, the innovative colored buffers included in the kit permit error-free visualization identification of complete bacterial cell lysis and neutralization.

Our Zyppy™ Plasmid Miniprep Kit is the fastest and simplest method available to efficiently separate plasmid DNA from E. coli. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in bacterial transformation, restriction endonuclease digestion (below), DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications. An overview of the purification procedure is shown to the upper right.