CDCA2,细胞分裂周期蛋白2抗体

产品编号HZ-7786R
英文名称CDCA2
中文名称细胞分裂周期蛋白2抗体
别 名cell division cycle associated 2; Recruits PP1 onto mitotic chromatin at anaphase protein; Repo Man; Repo-Man; CDCA2_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 细胞周期蛋白 细胞分化 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,
CDCA2,细胞分裂周期蛋白2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量113kDa
细胞定位细胞核 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CDCA2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CDCA2,细胞分裂周期蛋白2抗体PubMedPubMed
产品介绍background:
CDCA2 is a regulator of chromosome structure during mitosis. CDCA2 forms an essential complex with protein phosphatase PP1-gamma subunit 1 (PPP1CC) and is required for the recruitment of PP1 to chromatin. Knockdown of CDCA2 by RNAi causes large-scale cell death by apoptosis, as does overexpression of a dominant-negative mutant of the protein.

Function:
Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.

Subunit:
Interacts with PPP1CC.

Subcellular Location:
Nucleus. Note=Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q69YH5-1; Sequence=Displayed; Name=2; IsoId=Q69YH5-2; Sequence=VSP_025598, VSP_025599; Note=No experimental confirmation available.

CDCA2,细胞分裂周期蛋白2抗体Tissue Specificity:
Ubiquitously expressed.

Post-translational modifications:
Phosphorylated by CDK1. May regulate its subcellular location.

Database links:
UniProtKB/Swiss-Prot: Q69YH5.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CDCA2 Polyclonal Antibody, Unconjugated(HZ-7786R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining