上皮间充质转化(EMT)ChIP qPCR芯片用于研究上皮间充质转变(EMT)的84个关键基因的组蛋白修饰状态或“组蛋白密码”。组蛋白修饰调节染色质结构,从而对基因表达的表观遗传调控发挥关键作用。EMT和相互间质上皮过渡（MET）是参与肿瘤的转移和干细胞分化和发育的关键步骤。在EMT期间，上皮细胞失去他们的顶点性和基底极性，破坏其细胞间的紧密连接，降解基底膜细胞外基质成分成为可迁移的间质细胞。因此该芯片包括细胞表面受体，细胞外基质，细胞骨架基因介导的细胞黏附、迁移、运动和形态，控制细胞分化、发育、生长和增殖的基因，以及导致EMT和所有相关的进程的信号转导和转录因子基因。结果可助于进一步洞察组蛋白修饰在调节EMT依赖性肿瘤转移和干细胞分化和发展中的作用。利用这个芯片通过染色质免疫沉淀和实时定量PCR，可以很简易、可靠地分析组蛋白的化学修饰模式与EMT相关重要基因的关联。

EpiTect ChIP qPCR仅用于分子生物学应用。本产品不用于疾病的诊断、预防和治疗。

Genes Up-Regulated During EMT: AHNAK, BMP1, CALD1, CDH2 (N-cadherin), COL1A2, COL3A1, COL5A2, FN1, FOXC2, GNG11, GSC, IGFBP4, ITGA5, ITGAV, MMP2 (Gelatinase A), MMP3, MMP9 (Gelatinase B), MSN, SERPINE1 (PAI-1), SNAI1, SNAI2, SNAI3, SOX10, SPARC, STEAP1, TCF4, TIMP1, TMEFF1, TMEM132A, TWIST1, VCAN, VIM, VPS13A, WNT5A, WNT5B.

Genes Down-Regulated During EMT: CAV2, CDH1 (E-cadherin), DSP, FGFBP1, IL1RN, KRT19, MITF, MST1R, NUDT13, PPPDE2, RGS2, SPP1 (Osteopontin), TFPI2, TSPAN13.

Genes with Known Histone Modifications during EMT:
Increased H3K4me3: AHNAK, AKT1, BMP1, CALD1, CAV2, CDH2 (N-cadherin), CTNNB1, FN1, FZD7, GNG11, GSK3B, IGFBP4, ILK, ITGA5, MAP1B, MITF, MMP2 (Gelatinase A), RGS2, SERPINE1 (PAI-1), SNAI1, SNAI2, SPARC, TCF4, TGFB1, TGFB2, TGFB3, TIMP1, TMEFF1, TSPAN13, VIM, VPS13A, WNT5A.
Decreased H3K27me3: DSP, FGFBP1, GSC, IL1RN.

Differentiation & Development: AKT1, BMP1, BMP2, BMP7, COL3A1, COL5A2, CTNNB1, DSP, ERBB3, F11R, FOXC2, FZD7, GSC, KRT14, MITF, MST1R, NODAL, NOTCH1, PTP4A1, SMAD2, SNAI1, SNAI2, SOX10, TGFB2, TGFB3, TMEFF1, TWIST1, VCAN, WNT11, WNT5A, WNT5B.

Morphogenesis: CTNNB1, FOXC2, PPP3R1, RAC1, SMAD2, SNAI1, SOX10, TGFB1, TGFB2, TGFB3, TWIST1, WNT11, WNT5A.

Cell Growth & Proliferation: AKT1, BMP1, BMP2, BMP7, CAV2, CTNNB1, EGFR, ERBB3, FGFBP1, FOXC2, HIF1A, IGFBP4, ILK, MST1R, NODAL, PDGFRB, TGFB1, TGFB2, TGFB3, TIMP1, VCAN.

Migration & Motility: CALD1, CAV2, EGFR, FN1, ITGB1, MSN, MST1R, NODAL, PDGFRB, RAC1, STAT3, TGFB1, VIM.

Cytoskeleton: CAV2, KRT7, MAP1B, PLEK2, RAC1, VIM.

Extracellular Matrix & Cell Adhesion: BMP1, BMP2, BMP7, CDH1 (E-cadherin), CDH2 (N-cadherin), COL1A2, COL3A1, COL5A2, CTGF, CTNNB1, DSC2, EGFR, ERBB3, F11R, FN1, FOXC2, ILK, ITGA5, ITGAV, ITGB1, MMP2 (Gelatinase A), MMP3, MMP9 (Gelatinase  B), PTK2, RAC1, SERPINE1 (PAI-1), SPP1 (Osteopontin), TGFB1, TGFB2, TIMP1, VCAN.

Signaling Pathways:
Estrogen Receptor: CAV2, ESR1 (ERa), KRT19, TGFB3.
G-Protein Coupled Receptor: AKT1, FZD7, GNG11, RAC1, RGS2.
Integrin-Mediated: COL3A1, CTGF, ILK, ITGA5, ITGAV, ITGB1, PTK2.
Notch: FOXC2, NOTCH1.
Receptor Tyrosine Kinase: EGFR, ERBB3, PDGFRB, RGS2, SPARC.
TGFb / BMP: BMP1, BMP2, BMP7, COL3A1, SMAD2, SMAD4, TGFB1, TGFB2, TGFB3.
WNT: CTNNB1, FZD7, GSK3B, WNT11, WNT5A, WNT5B.

Transcription Factors: CTNNB1, ESR1 (ERa), FOXC2, GSC, MITF, NOTCH1, SIP1, SMAD2, SNAI2, SNAI3, SOX10, STAT3, TCF4, TWIST1, ZEB2