纯化His标签蛋白试剂盒-蛋白纯化-试剂-生物在线
纯化His标签蛋白试剂盒

纯化His标签蛋白试剂盒

商家询价

产品名称： 纯化His标签蛋白试剂盒

英文名称： Protino Ni-TED Resin (600 g)Protino Ni-TED 树脂

产品编号： 745200.600

产品价格： 0

产品产地： 德国

品牌商标： MN(Macherey-Nagel)

更新时间： null

使用范围： null

广州伟杰生物科技有限公司
  • 联系人 :
  • 地址 : 广州市荔湾区西湾路广雅前街2号荔海商务大厦310室
  • 邮编 :
  • 所在区域 : 广东
  • 电话 : 189****9166
  • 传真 :
  • 邮箱 : weijiebio@189.cn
进入展台

 

Protino® Ni-TED Resin

Purification of His-tag proteins
 

Features
 

Protino® Ni-TED, the pure alternative for His-tag protein purification

 

 

 

• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding 
   site provides highest selectivity and purity (also see Technologies)
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins 
   compared to other common IMAC matrices
• Dry resin – storage at room temperature

Protino® Ni-TED Resin
• Dry bulk material
• Rigid support silica matrix, compatible with high pressure and high flow rates, 
   does not shrink or swell – highest flexibility
• Batch procedure, gravity-flow column chromatography, FPLC™ applications
• Empty Protino® columns available for use with Protino® Ni-TED Resin
• Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin
   
Technology IMAC (immobilized metal ion affinity chromatography)
Chelating ligand TED (tris-carboxymethyl ethylene diamine)
Format Bulk matrix
Matrix Macroporous silica

Physical form

 Dry matrix, precharged with Ni2+

Binding capacity*

10 mg/g resin
5 mg/mL bed volume
Max. pressure 145 psi (10 bar)
* Binding capacity will vary for each polyhistidin-tagged protein
 
Applications
 
• Purification of polyhistidine-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications
  

      Maximize        
 
 


     

Ordering information

Product

Preps

 Specification REF
Protino® Ni-TED Resin

5 g

 dry silica bulk resin 745200.5
 

30 g

 as above 745200.30
 

120 g

 as above 745200.120
 

600 g

 as above 745200.600
       
Product accessories

Pack of

 Specification REF
Protino® Columns 14 mL

10

 empty columns (gravity flow) for use with e.g. Protino® Ni-TED / IDA Resin 745250.10
Protino® Columns 35 mL

10

 empty columns (gravity flow) for use with e.g. Protino® Ni-TED / IDA Resin 745255.10
Receiver Plate 50 µm hydrophilized

4

 96-well plates with an inserted hydrophilized filter frit of 50 µm pore size, suitable for gravity flow, centrifugation, and vacuum 740689.4