Apoptosis was induced in Jurkat cells by incubating cells with 2 µM Camptothecin for 5 hours at 37°C (Lane 2) and DNA was visualized using the DNA ladder kit. DNA from Jurkat cells without Camptothecin treatment were used as control (Lane 1). 

描述

The ApoTarget™ Quick Apoptotic DNA Ladder Detection Kit provides a simple and rapid procedure for extraction of chromosomal DNA. The procedure prepares DNA for use in the methods that detect DNA fragmentation in apoptotic cells. Unlike other kits requiring 1 to 2 days to finish, this detection method requires only less than 90 minutes to prepare DNA in a single tube without the need for extraction or column steps. DNA fragmentation can be easily visualized by agarose gel electrophoresis. This procedure increases recovery of small fragmented DNA, thereby improving the sensitivity of the assay.

内容和储存

Contains TE lysis buffer, enzymes A and B, ammonium acetate solution, and DNA suspension buffer. Store kit at -20°C.

详细说明

常用规格