cfRNA分离纯化试剂盒
产品名称： cfRNA分离纯化试剂盒
英文名称： NextPrep™ Magnazol™ cfRNA Isolation Kit
产品编号： NOVA-3830-01
产品价格： 0
产品产地： 进口
品牌商标： BIOO SCIENTIFIC
更新时间： 2023-09-20T19:30:01
使用范围： null
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- 所在区域 : 上海
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- 邮箱 : 1481397330@qq.com
Extracting cfRNA from Human Plasma or Serum
The NextPrep™ Magnazol™ cfRNA isolation kit is designed for extracting cell-free RNA (cfRNA) for constructing NGS libraries from human blood plasma or serum, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cfRNA is ideal for next generation sequencing. Suitability of the RNA for use in NGS library construction has been verified using the NEXTFLEX® small RNA-Seq v3 library prep kit for Illumina® sequencing.
产品特点：
Ideal for extracting cfRNA from human plasma or serum for small RNA-seq
Magnetic beads for rapid purification of RNA from aqueous phase
Allows highest volume of biofluids to be processed in a single tube
Fast, < 1 hour protocol with minimal hands-on time
Generates quality NGS libraries using few PCR cycles
No loss of pellet during IPA precipitation
Kit includes BCP, an alternative to chloroform
All steps carried out at room temperature
产品列表：
| 货号 | 产品名称 | 规格 |
| NOVA-3830-01 | NextPrep™ Magnazol™ cfRNA Isolation Kit | 25 ISOLATIONS |
| NOVA-5132-05 | NextPrep™ Magnazol™ cfRNA Isolation Kit （SMALL RNA-SEQ） | 8 RXNS |
| NOVA-5132-06 | NextPrep™ Magnazol™ cfRNA Isolation Kit （SMALL RNA-SEQ） | 48 RXNS |
Cell Free RNA Isolation Protocol
This cell free RNA isolation procedure, which can be completed in less than 1 hour, includes an initial lysis/RNase inactivation, followed by phase separation, magnetic bead-based RNA binding step, wash steps, and elution of cfRNA from the magnetic beads.
Cell Free RNA Isolation Challenges
Extraction of cfRNA from plasma is challenging because it is highly heterogeneous in size and present in low concentrations. Since the expected yields are low, the RNA recovered cannot be reliably detected using standard methods such as NanoDrop spectrophotometer, gel or capillary electrophoresis, or RNA binding dyes. The NextPrep™ Magnazol™ cfRNA isolation kit allows for the highest volume of biofluids to be processed in a single microfuge tube compared to other kits on market.
The NextPrep™ Magnazol™ cfRNA isolation kit delivers robust cfRNA yields and quality NGS libraries with a high percentage of reads that map to miRbase than other kits on market and allows for miRNA diversity exploration using the NEXTFLEX® small RNA-Seq v3 kit.
KIT CONTENTS
Magnazol™ Extraction Reagent
Magnazol™ Magnetic Beads
BCP (1-bromo-3-chloropropane)
RNA Elution Solution (0.1 mM EDTA)
REQUIRED MATERIALS NOT PROVIDED
Ethanol, 100% and 80%
Vortex mixer
Platform shaker, tube rotator, or heat block
Microcentrifuge or “picofuge”
2 mL microfuge tubes, nuclease-free. Smaller tubes may be used for the elution step.
Pipettors (P-1000, P-200, P-20 or equivalent) and tips
Magnetic stand for microfuge tubes.