抗His 单克隆抗体
产品名称: 抗His 单克隆抗体
英文名称: Anti-His antibody
产品编号: BF-His.H8
产品价格: null
产品产地: 美国
品牌商标: BioFront
更新时间: null
使用范围:
- 联系人 :
- 地址 : 安徽省合肥市经开区凤凰国际2号楼
- 邮编 : 230601
- 所在区域 : 安徽
- 电话 : 139****2696
- 传真 : 0551-63843599
- 邮箱 : info@biofrontchina.com
抗HHHHHH (6x His)标签(HIS.H8)单克隆抗体 |
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Clone:HIS.H8 克隆:HIS.H8 |
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Volume:100ul、500ul、(1 ug/ul) 体积:100ul、500ul、(1 ug/ul) |
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Description:Anti-HHHHHH (6x His) Tag (HIS.H8) Monoclonal Antibody 描述:抗HHHHHH (6x His)标签(HIS.H8)单克隆抗体 |
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Clonality |
Host Species |
Isotype |
HIS.H8 |
Mouse |
IgG2b |
克隆类型 |
宿主 |
同种型 |
HIS.H8 |
小鼠 |
IgG2b |
Immunogen:HHHHHH (6x His) synthetic peptide conjugated to KLH 免疫原:与血蓝蛋白(KLH)偶联的HHHHHH (6x His)合成肽 |
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Specificity:Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags) 特异性:识别多种市售的载体编码的6x His-tags表位,不论标签在融合蛋白序列中的定位(即:与6x His-tags的N端,C端或内部反应) |
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Purification:Protein A affinity chromatography from mouse ascites fluid 纯化:小鼠腹水经过A蛋白亲和层析 |
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Application:Dot, ELISA, IP, IS, WB 应用:点杂交(Dot),酶联免疫吸附实验(ELISA),免疫沉淀(IP),IS,免疫印迹(WB) |
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Sensitivity:WB (with ECL): 1-2ng of His-tagged fusion proteins 敏感度:WB(ECL):1-2ng His标记融合蛋白 |
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Working Condition: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test 工作条件: WB(ECL):1:1000-3000稀释(室温孵育1小时) IS:1:500-2000稀释 为了获得其他实验的最佳结果(如:点杂交,酶联免疫吸附,免疫沉淀,等等),请做滴度测定来确定最理想的工作稀释度 |
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Storage:Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C (add 0.05% NaN3) for several days to weeks 存储:-20°C可以存放2年或更长时间。第一次解冻后请离心以得到最大产品回收。分装成小份以避免反复冻融周期。将小份存储在4°C(加0.05% NaN3)可以存放几天至几周。 |
Images
图像
Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
图01:1:1000 (1μg/mL)抗体稀释探测转染HA标记蛋白载体的;未转染(1),转染(2)
左图:未转染对照;右图:anti-His(红色)在HEK293细胞中His标记融合蛋白之上。对比染色剂:DAPI(蓝色)
Fig. 02 Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5μg/mL)
图02:与两个不同供应商提供的抗His标签(HIS.H8)单抗的比较,探测标准Ladder(Qiagen Cat. No. 34705),其包含5种不同的His标记蛋白。所有抗体稀释度1:2000(0.5μg/mL)
Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)
图03:标准Ladder(含有5种不同的His标记蛋白);未转染对照(1),His标记蛋白载体转染的HEK293细胞(2)
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Item #: His.H8-100 or His.H8-500 or His.H8-1