蛋白信号调节因子6(RGS6)重组蛋白

YB706Ra013
Recombinant Regulator Of G Protein Signaling 6 (RGS6)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
蛋白信号调节因子6(RGS6)重组蛋白[ PROPERTIES ]
Residues: Met1~Ser472
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: F1LS67
Host: E. coli
Subcellular Location: Cytoplasm. Membrane.
Peripheral membrane protein.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
蛋白信号调节因子6(RGS6)重组蛋白150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.1
Predicted Molecular Mass: 58.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
蛋白信号调节因子6(RGS6)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MAQGSGDQRA VGVADPEESS PNMIVYCKIE DIITKMQDDK TGGVPIRTVK SFLSKIPSVV
TGTDIVQWLM KNLSIEDPVE AIHLGSLIAA QGYVFPISDH VLTMKDDGTF YRFQAPYFWP
SNCWEPENTD YAIYLCKRTM QNKARLELAD YEAENLARLQ RAFARKWEFI FMQAEAQVKI
DRKKDKTERK ILDSQERAFW DVHRPVPGCV NTTEMDIRKC RRLKNPQKVK KSVYGVTDES
QSQSPVHIPS QPIRKTTKDD IRKQITFLNA QIDRHCLKMS KVAESLIAYT EQYVEYDPFI
TPAEPSNPWI SDDITLWDIE MSKEPSQQRV KRWGFSFDEI LKDQVGRDQF LRFLESEFSS
ENLRFWLAVQ DLKKQPLQDV AKRVEEIWQE FLAPGAPSAI NLDSHSYEIT SQNVKDGGRY
TFEDAQEHIY KLMKSDSYAR FLRSNAYQDL LLAKKKGKSL AGKRLTGLMQ SS