phospho-NCF1 (Ser359),磷酸化嗜中性粒细胞胞浆因子1抗体

产品编号HZ-11445R
英文名称phospho-NCF1 (Ser359)
中文名称磷酸化嗜中性粒细胞胞浆因子1抗体
别 名phospho-NCF1(Ser359); phospho-p47 phox(Ser359); NCF1 (phospho S359); 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; NOXO2; p47 phox; p47phox; ;SH3 and PX domain containing protein 1A; SH3PXD1A; NADPH oxidase p47 phox; NCF1_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Rat,
phospho-NCF1 (Ser359),磷酸化嗜中性粒细胞胞浆因子1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-NCF1 (Ser359),磷酸化嗜中性粒细胞胞浆因子1抗体PubMedPubMed
产品介绍background:
The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.

Function:
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Subunit:
Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.

DISEASE:
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

Similarity:
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains.

phospho-NCF1 (Ser359),磷酸化嗜中性粒细胞胞浆因子1抗体Database links:
Entrez Gene: 281345 Cow
Entrez Gene: 653361 Human
Entrez Gene: 17969 Mouse
Entrez Gene: 100134857 Pig
Entrez Gene: 100008803 Rabbit
Entrez Gene: 114553 Rat
Omim: 608512 Human
SwissProt: O77774 Cow
SwissProt: P14598 Human
SwissProt: Q09014 Mouse
Unigene: 647047 Human
Unigene: 655201 Human
Unigene: 425296 Mouse
Unigene: 38575 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.