2X TaqMan Fast qPCR 预混液（低ROX）

附带条件

特技

2xTaqman快速qPCR主混合（Low Rox）是一种随时可用的鸡尾酒，含有快速热启动Taq DNA聚合酶、MgSO4、DNTP混合物和一些稳定剂。它提供了一个2X主混合物包含所有成分，除了引物，探针和模板快速循环探针实时PCR。快速热启动Taq DNA聚合酶和专有缓冲系统减少了引物二聚体的形成，增强了实时qPCR扩增特异性和高检测灵敏度。DNF缓冲液对存在多个非特异性扩增的引物是特异性的。对于一些DNA序列没有良好的引物，DNF缓冲液可以按照以下方案加入到反应混合物中。ROX参考染料在2xTaqMn快速qPCR主混合（Low Rox）中为100 nm，适用于需要由低浓度ROX参考标准化的实时PCR仪器，如应用生物系统7500，应用生物系统7500快速，战略MX3000 0P。，战略MX300 5P？战略MX4000？应用生物系统VIIA- 7和应用生物系统QuangStudio？12K屈曲。

特技

2×TaqMan快速qPCR大师混合物是一种现成的鸡尾酒，它只添加引物探针和模板来建立反应混合物。

热启动Taq DNA聚合酶的独特热启动方式和最佳缓冲体系确保了扩增的特异性和高效性。

在2×TaqMan快速qPCR主混合中，热启动Taq DNA聚合酶具有比普通热启动Taq DNA聚合酶更快的延伸率，使得实时qPCR的总反应时间更短。

独特的DNF缓冲液对存在多个非特异性扩增的引物具有特异性。

共有三种2×SG快速qPCR主混合物，包括NO /低/高ROX参考，适用于所有的实时PCR仪器。

船首

一个词

特赦

2XTAQMAN快速qPCR主混（Low Rox）；DNF缓冲液；灭菌DDH2O。

概述

The 2xTaqMan Fast qPCR Master Mix(Low Rox) is a ready-to-use cocktail containing fast hot start Taq DNA Polymerase, MgSO4, dNTP Mix and some stabilizers. It is supplied as a 2X Master Mix containing all components except primers, probe and template for fast-cycling probe-based real-time PCR. The fast hot start Taq DNA Polymerase and proprietary buffer system reduce the formation of the primer dimers, enhance the amplification specificity and high detection sensitivity in real-time qPCR. The DNF Buffer is specific for the primers which exist multi nonspecific amplification. For some DNA sequences there are no good primers, the DNF Buffer can be added to the reaction mix according to the following protocol. The ROX reference dye is 100 nM in the 2xTaqMan Fast qPCR Master Mix(Low Rox) and applicable to the real time PCR instruments that are need to normalization by the low concentration of Rox reference, like Applied Biosystems 7500, Applied Biosystems 7500 Fast, Stratagene Mx3000P?, Stratagene Mx3005P?, Stratagene Mx4000?, Applied Biosystems ViiA 7 and Applied Biosystems QuantStudio? 12K Flex.

特点

• The 2×TaqMan Fast qPCR Master Mix is a ready-to-use cocktail, which only add primers probe and template to set up the reaction mix.
• The unique hot-start way of the hot start Taq DNA Polymerase and optimal buffer system ensure the high amplification specificity and efficiency.
• The hot start Taq DNA Polymerase in the 2×TaqMan Fast qPCR Master Mix has faster extension rates than common hot start Taq DNA Polymerase，making the total reaction time of the real time qPCR shorter.
• The unique DNF Buffer is specific for the primers which exist multi nonspecific amplification.
• There are three kinds of 2×SG Fast qPCR Master Mix including no/low/high Rox reference，applying to all the the real time PCR instruments.

应用

基因表达分析、拷贝数分析

组份

2xTaqMan Fast qPCR Master Mix(Low Rox); DNF Buffer; Sterilized ddH2O.