迟发型超敏反应-科研实验整体外包服务-技术服务-生物在线
迟发型超敏反应

迟发型超敏反应

商家询价

产品名称: 迟发型超敏反应

英文名称: DTH, Delayed Type Hypersensitivity

产品编号: BDA-IF004

产品价格: 询价

产品产地: null

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上海百代安生物科技有限公司
  • 联系人 :
  • 地址 : 上海市浦东新区张江高科蔡伦路720号1号楼413室
  • 邮编 : 201203
  • 所在区域 : 上海
  • 电话 : 186****6750
  • 传真 : 021-38751963
  • 邮箱 : kyh@gmail.com

迟发型超敏反应
实验方案:
1) ICR小鼠随机分组后,1%三硝基氯苯腹部致敏6天;
2) 地塞米松给药6天;
3) 6天后, 1%三硝基氯苯刺激ICR耳朵;
4) 24小时后打耳片称重,取脾脏,胸腺称重;分别计算耳肿胀程度和脾脏,胸腺指数;

Brief protocol of PC induced Delayed Type Hypersensitivity (DTH)
1) Mice were sensitized by applying 100u l of 1% PC dissolved in ethanol to the abdominal skin.
2) After 6 d, animals were challenged by painting 30u l of1% PC dissolved in castor oil on right ear lobes.
3) 24 h after challenge, the mice were sacrificed, the left and right ears were removed at the same location and weighted. Ear swelling
was represented as the weight difference between right lobes and left ones.
4) Spleen and thymus were also collected and weighted for the spleen index and thymus index.