β-血小板球蛋白(βTG)重组蛋白

YB370Ra021
Recombinant Beta-Thromboglobulin (bTG)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
β-血小板球蛋白(βTG)重组蛋白[ PROPERTIES ]
Residues: Ala59~Asp128 (Accession # P02775),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
β-血小板球蛋白(βTG)重组蛋白0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.3
Predicted Molecular Mass: 11.2kDa
Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in ddH2O. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
β-血小板球蛋白(βTG)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- AE LRCMCIKTTS GIHPKNIQSL
EVIGKGTHCN QVEVIATLKD GRKICLDPDA PRIKKIVQKK LAGDESAD
[ REFERENCES ]
1. Wenger R.H., et al. (1989) Blood 73:1498-1503. 2. Majumdar S., et al. (1991) J. Biol. Chem. 266:5785-5789. 3. Zhang C., et al. (2001) Blood 98:610-617. 4. Holt J.C., et al. (1986) Biochemistry 25:1988-1996.