EIF3D抗体-抗体-抗体-生物在线
EIF3D抗体

EIF3D抗体

商家询价

产品名称： EIF3D抗体

英文名称： EIF3DAntibody

产品编号： XY10219-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com
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 EIF3D抗体

Applications
In-house applications ELISA, IF, IHC, WB     Application not tested? Please see our Guarantee.
Published applications IF, IP, WB Publications 6 citations –view here
Species specificity Human, Mouse, Yeast; other species not tested.
Published Species Human, mouse, rabbit, yeast
Images
Prev12NextView more validation data
  • 10219-1-AP;mouse brain tissuemouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP( EIF3D Antibody) at dilution of 1:600
  • 10219-1-AP;N/AF result of 10219-1-AP(anti-EIF3D) in U2OS cell (treated with 100 mM sodium arsenite to cause stress-induced translational arrest) by Dr. Nancy Kedersha.U2OS cells (FAST-YFP stables; but not showing FAST-YFP); stained with PTG anti-eIF3p66 in green; and counterstained with anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.
  • 10219-1-AP;MCF-7Immunofluorescent analysis of MCF-7 cells, using EIF3D antibody 10219-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 10219-1-AP;human breast cancer tissueImmunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10219-1-AP( EIF3D Antibody) at dilution of 1:50 (under 40x lens)
Positive WB detected in Mouse brain tissue, A549 cells, HepG2 cells, human brain tissue, L02 cells
Positive IHC detected in Human breast cancer tissue
Positive IF detected in MCF-7 cells, Ethacrynic acid treated HepG2 cells, HepG2 cells, transfected cells
Recommended dilution WB : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200
 EIF3D抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen EIF3D fusion protein ag0268 Full name Eukaryotic translation initiation factor 3, subunit D
Calculated molecular
weight		 66 kDa Observed molecular 
weight		 66 kDa
GenBank accession 
number		 BC000328 Gene ID (NCBI) 8664
Gene symbol EIF3D Synonyms EIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7
Background EIF3D抗体

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine. EIF3D抗体

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