Palladin 抗体-抗体-抗体-生物在线
Palladin 抗体

Palladin 抗体

商家询价

产品名称： Palladin 抗体

英文名称： Palladin Antibody

产品编号： XY10853-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com
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 PALLADIN 抗体Applications

In-house applications ELISA, WB, IP, IHC, FC, IF     Application not tested? Please see our Guarantee.
Published applications IF, IHC, IP, WB Publications 36 citations –view here
Species specificity Human, Mouse; other species not tested.
Published Species Human, mouse, rat
Images
Prev123NextView more validation data
  • 10853-1-AP;A549 cellsA549 cells (shcontrol and shRNA of PALLD) were subjected to SDS PAGE followed by western blot with 10853-1-AP (PALLD antibody) at dilution of 1:2000. (Data provided by Angran Biotech (www.miRNAlab.com)).
  • 10853-1-AP;human colon cancer tissueImmunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10853-1-AP( PALLD,palladin Antibody) at dilution of 1:50 (under 10x lens)
  • 10853-1-AP;HeLa cellsIP Result of anti-PALLD,palladin (IP:10853-1-AP, 4ug; Detection:10853-1-AP 1:2000) with HeLa cells lysate 1800ug.
  • 10853-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 10853-1-AP(PALLD,palladin antibody) at dilution of 1:2000
  • 10853-1-AP;human colon cancer tissueImmunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10853-1-AP( PALLD,palladin Antibody) at dilution of 1:50 (under 40x lens)
  • 10853-1-AP;HeLa cellsImmunofluorescent analysis of HeLa cells using 10853-1-AP( PALLD,palladin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 10853-1-AP;HeLa cells1X10^6 HeLa cells were stained with 0.2ug PALLD,palladin antibody (10853-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in HeLa cells, A549 cells, HepG2 cells, mouse kidney tissue
Positive IP detected in HeLa cells, HEK-293 cells
Positive IHC detected in Human colon cancer tissue, human breast cancer tissue, human colon tissue, human ovary tumor tissue
Positive IF detected in HeLa cells
Positive FC detected in HeLa cells
Recommended dilution WB : 1:1000-1:10000
IP : 1:1000-1:10000
IHC : 1:20-1:200
IF : 1:20-1:200
 PALLADIN 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen Palladin fusion protein ag1288 Full name Palladin, cytoskeletal associated protein
Calculated molecular
weight		 1383aa, 151 kDa Observed molecular 
weight		 95 kDa, 140 kDa
GenBank accession 
number		 BC013867 Gene ID (NCBI) 23022
Gene symbol PALLD Synonyms CGI 151, FLJ22190, FLJ38193, FLJ39139, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002
Background PALLADIN 抗体

Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6. PALLADIN 抗体

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XY741Ra21 Dickkopf相关蛋白1(DKK1)单克隆抗体 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) Rattus norvegicus (Rat)

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