KATO III 人胃癌细胞
产品名称： KATO III 人胃癌细胞
英文名称： KATO III
产品编号： HTB-103
产品价格： 0
产品产地： 美国
品牌商标： ATCC
更新时间： null
使用范围： null
上海复祥生物科技有限公司
- 联系人 :
- 地址 : 上海市虹口区赤峰路630弄南雁大厦
- 邮编 :
- 所在区域 : 上海
- 电话 : 150****6580
- 传真 : 021-35080520
- 邮箱 : xiangfbio@163.com
HTB-103 KATO III 人胃癌细胞
| Organism | Homo sapiens, human |
|---|---|
| Tissue |
stomach:derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac
|
| Product Format | frozen |
| Morphology | spherical |
| Culture Properties | mixed, adherent and suspension |
| Biosafety Level | 1 |
| Disease | gastric carcinoma |
| Age | 55 years adult |
| Gender | male |
| Ethnicity | Asian HTB-103 KATO III 人胃癌细胞 |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | The stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected. |
|---|---|
| Clinical Data |
55 years adult
Asian
male
|
| Tumorigenic | Yes |
| Effects |
Yes, in cheek pouches of anti thymocyte serum treated hamsters
No, in nude mice
|
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. HTB-103 KATO III 人胃癌细胞 |
|---|---|
| Subculturing |
Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 2.. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 xg for 5 to10 minutes. 5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubator at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C HTB-103 KATO III 人胃癌细胞
|