PCR Array

心脏毒性PCR芯片可用于研究由药物或化合物诱导而发生心脏损伤的84个关键基因的表达。毒性反应是药物上市的检测指标之一，其中就包括心脏毒性反应的检测。出于安全考虑，在过去40年，约有10%的药品因为对心血管有影响而撤出临床市场。传统的形态学研究昂贵又耗时，且很难分辨是否为药物或化合物引发的心脏毒性反应。这一芯片包含了多个模型中由于药物或化合物引起的心脏损伤的生物标志物基因，既减少了实验周期和成本，也能在研究最初阶段就发现和剔除会引起心脏毒性反应的药物。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测心脏毒性相关基因的表达。
Up-Regulated: Muscle Contraction: KBTBD10. Angiogenesis: IL6, PLAU, SERPINE1, TGFB2. Signal Transduction: ABRA, ADRA2A, CCR1, CREM, IL6, RND1, SIK1, TGFB2, TIAM1. Cell Cycle: BCAT1, FOSL1, HSPA2, SIK1, TGFB2. Development: BTG2, CREM, EGR1, FHL1, HSPA2, IL6, RND1, SIK1, SPP1, TGFB2, TIAM1, TIMP1, UCP1, VCAN. Metabolism (Regulation & Mechanism): ABRA, ADRA2A, BTG2, CH25H, CREM, EGR1, FOSL1, IL6, PDK4, PLA2G4A, PUM2, RBM3, SIK1, TCF4, TGFB2, TIMP1. Migration / Motility: ABHD2, CCL7, CCR1, FOSL1, IL6, PLAU, PPBP, PVR, TGFB2. Other: CD14, CFD, CKM, FCGR2B, GPM6A, HAMP, KBTBD5, MT1F, PLUNC, REG3G, TUBB6, UCK2. Down-Regulated: Muscle Contraction: ACTA1, GJA1, KCNJ12, RPS6KB1. Angiogenesis: COL15A1, POSTN, VEGFA. Signal Transduction: AIFM1, DUSP8, GJA1, IGFBP5, ITPR2, RPS6KB1, S1PR2, THRAP3. Cell Cycle: CSNK2A2, MCM6, PSMA2, PSMD7, TXNIP. Development: ACTA1, COL15A1, COL3A1, GJA1, IGFBP5, PLN, RPS6KB1, SOX4, TXNIP. Metabolism (Regulation & Mechanism): ASH1L, ATP5J, DUSP8, IGFBP5, MCM6, NFIB, PPP1R14C, PRKAB2, S1PR2, SOX4, THRAP3, TXNIP, ZNF148, ZNF23. Migration / Motility: IGFBP5, NEXN, RPS6KB1. Other: AK3, BGN, BSN, HSPH1, IFT20, PKN2, SLC4A3, UBA5, UBXN2A, VIM, WIPI1