Product Highlight

• Configuration-specific monoclonal antibody that recognizes Rab7-GTP, but not Rab7-GDP
• The only available Rab7 activation assay kit directly measures the level of Rab7-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Rab7 in cells and in tissues by immunohistochemistry

Product Description

Rab7 belongs to the Rab family of small GTPases. Rab7 is located in the late endosome, lysosome, cytoplasmic vesicle, phagosome and melanosome. It is involved in the late endocytic transport and contributes to the maturation of phagosomes.

Currently there is no direct assay to measure the activation of Rab7 GTPases.

NewEast Biosciences Rab7 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab7-GTP, but not Rab7-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti- Rab7-GTP monoclonal antibodies can also be used to monitor the activation of Rab7 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Rab7 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab7. Each kit provides sufficient quantities to perform 20 assays.

 

Assay Principle

NewEast Biosciences Rab7 Activation Assay Kit bases on the configuration-specific anti-Rab7-GTP monoclonal antibody to measure the active Rab7-GTP levels, either from cell extracts or from in vitro GTPγS loading Rab7 activation assays. Briefly, anti-active Rab7 mouse monoclonal antibody will be incubated with cell lysates containing Rab7-GTP. The bound active Rab7 will then be pulled down by protein A/G agarose. The precipitated active Rab7 will be detected by immunoblot analysis using anti- Rab7 rabbit polyclonal antibody.

Publications:

1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors     Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2.  Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance     Endocrinology. 2014 Sep;155(9):3315-28