BstPAI-核酸/蛋白合成-试剂-生物在线

产品名称： BstPAI

英文名称： BstPAI

产品编号： HZ600410

产品价格： 0

产品产地：中国/上海

品牌商标： HZbscience

更新时间： 2023-08-17T10:24:20

使用范围： null

上海沪震实业有限公司

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BstPAI

产品描述

使用说明

高浓度和孵化酶在150度16 hours for results in星活动。

技术规格

Unit One Unit of the definition酶水解is required to the amount ofλDNA 1μg in at 1小时150度总量在50μl反应。

网站gacnn↑nngtc recognition

stearothermophillus源芽孢杆菌PA

我们assayedλDNA

优化的缓冲区（33毫米和醋酸（pH 7.9毫米（10°C）；醋酸醋酸钾镁；66毫米；1毫米DTT。）

温度150度优化

10毫米的存储条件的Tris-HCl（pH 7.5）；50毫米氯化钾0.1毫米；EDTA；200μg／ml）；7毫米2 -巯基乙醇；50%的甘油。商店在零下20摄氏度。

ligations after 5倍小于5% overdigestion酶与DN**段ligated can be of the

非特异性水解后没有检测到nonspecific孵化of activity was with 10λDNA 1μg of hours of酶在UA听说是八周16°C

甲基化效应不灵敏的测试

热灭活不

在pshai campatible渤西，

产品描述

使用说明

High enzyme concentration and incubation at 65°C for 16 hours results in star activity.

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Recognition sites	GACNN↑NNGTC
Source	Bacillus stearothermophillus PA
Assayed on	Lambda DNA
Optimal buffer	Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature	65°C
Storage conditions	10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations	After 5-fold overdigestion with enzyme less than 5% of the DNA fragments can be ligated
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 25°C
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	NO
Campatible Ends	BoxI, PshAI