泛素特异性肽酶7(USP7)重组蛋白

YB255Hu014
Recombinant Ubiquitin Specific Peptidase 7 (USP7)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
泛素特异性肽酶7(USP7)重组蛋白[ PROPERTIES ]
Residues: Glu54~Glu507
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q93009
Host: E. coli
Subcellular Location: Nucleus. Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
泛素特异性肽酶7(USP7)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.7
Predicted Molecular Mass: 56.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
泛素特异性肽酶7(USP7)重组蛋白that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
EATFQFT VERFSRLSES VLSPPCFVRN LPWKIMVMPR FYPDRPHQKS VGFFLQCNAE
SDSTSWSCHA QAVLKIINYR DDEKSFSRRI SHLFFHKEND WGFSNFMAWS EVTDPEKGFI
DDDKVTFEVF VQADAPHGVA WDSKKHTGYV GLKNQGATCY MNSLLQTLFF TNQLRKAVYM
MPTEGDDSSK SVPLALQRVF YELQHSDKPV GTKKLTKSFG WETLDSFMQH DVQELCRVLL
DNVENKMKGT CVEGTIPKLF RGKMVSYIQC KEVDYRSDRR EDYYDIQLSI KGKKNIFESF
VDYVAVEQLD GDNKYDAGEH GLQEAEKGVK FLTLPPVLHL QLMRFMYDPQ TDQNIKINDR
FEFPEQLPLD EFLQKTDPKD PANYILHAVL VHSGDNHGGH YVVYLNPKGD GKWCKFDDDV
VSRCTKEEAI EHNYGGHDDD LSVRHCTNAY MLVYIRE