LightCycler ® Multiplex DNA Master   双杂交探针®多重DNA大师

Easy-to-use 5x reaction mix optimized for multiplex qPCR, compatible with the LightCycler® 480 or the LightCycler® 96 Real-Time PCR Systems.

易于使用的5倍反应混合优化的多重qPCR，与LightCycler®480或LightCycler®96实时PCR系统兼容。

For general laboratory use.

供一般实验室使用。

Product No：07339577001   产品编号：07339577001  

Pack Size：1000 x 20 μL reactions  包装尺寸：1000 x 20 μL 反应

Product No：07339585001  产品编号：07339585001   

Pack Size：200 x 20 μL reactions   包装尺寸：200 x 20 μL 反应

Keywords: Pathogen Detection  关键词：病原体检测

Overview概述：

Rely on this new Real-Time PCR Master Mix for fast and highly sensitive multiplex detection of DNA targets and internal controls. Based on the aptamer-mediated hot start AptaTaq Fast DNA Polymerase and a 5x concentrated buffer formulation optimized for multiplex target detection, enabling sensitive qPCR of pathogen DNA targets isolated from a variety of human sample materials.

依靠这种新型 Real-Time PCR Master Mix 对 DNA 靶标和内部对照进行快速、高灵敏度的多重检测。基于适体介导的热启动 AptaTaq 快速 DNA 聚合酶和针对多重目标检测优化的 5 倍浓缩缓冲液配方，能够对从各种人类样本材料中分离的病原体 DNA 目标进行灵敏的 qPCR。

Perform fast, highly sensitive and specific qPCR detection of pathogen DNA targets isolated from a wide range of materials, including difficult sample materials (e.g., blood, plasma, serum, stool).

对从各种材料中分离的病原体 DNA 靶标进行快速、高灵敏度和特异性 qPCR 检测，包括难处理的样品材料（例如，血液、血浆、血清、粪便）.

Increase throughput and cost efficiency of the qPCR workflow with a master mix optimized for multiplex detection of up to 5 targets.

使用针对多达 5 个目标的多重检测而优化的预混液，提高 qPCR 工作流程的通量和成本效率.

Detect low copy number of DNA targets with higher sensitivity with a 5x master mix concentration

使用 5 倍预混液浓度以更高的灵敏度检测低拷贝数的 DNA 靶标

Decrease experiment set up time and effort with convenient single vial mix

使用方便的单瓶混合减少实验设置时间和精力

Automate your workflow or setup your reactions at room temperature with the thermostable enzyme in the master mix

使用预混液中的热稳定酶自动化您的工作流程或在室温下设置您的反应

Contents 内容

qPCR Reaction Mix, 5x conc.

qPCR 反应混合物，5x 浓度。

Water, PCR grade

水，PCR 级

Application应用：

The LightCycler® Multiplex DNA Master is a 5x master mix especially suited for the fast and efficient multiplex PCR detection of up to 5 DNA pathogen targets in human sample materials. The robust formulation allows multiplex qPCR detection of a multitude of pathogen targets without the need for reaction condition adaptation or titration of MgCl2 concentration. The PCR protocol has been designed to run on the LightCycler® 480 and LightCycler® 96 Systems. The master mix is optimized for use with hydrolysis probes as well as Universal ProbeLibrary (UPL) probes.

LightCycler ®  Multiplex DNA Master 是一种 5倍预混液，特别适用于对人类样品材料中多达 5 个 DNA 病原体靶标进行快速高效的多重 PCR 检测。稳健的配方允许多重 qPCR 检测多种病原体目标，而无需调整反应条件或滴定 MgCl 2 浓度。PCR 方案设计用于在 LightCycler ®  480 和 LightCycler ®  96 系统上运行。预混液经过优化，可与水解探针以及 Universal ProbeLibrary (UPL) 探针配合使用。