Anti-ATM Rabbit Polyclonal Antibody-抗体-抗体-生物在线
Anti-ATM Rabbit Polyclonal Antibody

Anti-ATM Rabbit Polyclonal Antibody

商家询价

产品名称： Anti-ATM Rabbit Polyclonal Antibody

英文名称： Anti-ATM Rabbit Polyclonal Antibody

产品编号： 21276

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
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  • 所在区域 : 湖北
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  • 邮箱 : neweast3@126.com
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ATM [21276]
[100 µL]		  

Catalog Number:  21276
Gene Symbol:  ATM
Description:   Anti-ATM Rabbit Polyclonal Antibody
Background:   Ataxia telangiectasia mutated (ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers.
Immunogen:   Purified ATM protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:200
Concentration:    0.2mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes ATM of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant ATM protein. Purified His-tagged ATM protein was blotted with anti-ATM rabbit polyclonal antibody (Cat. #21276).
Immunohistochemistry:  
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-ATM polyclonal antibody (Cat. #21276). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody