ProSAPiP1,含脯氨酸突触相关蛋白相互作用蛋白1抗体

产品编号HZ-11198R
英文名称ProSAPiP1
中文名称含脯氨酸突触相关蛋白相互作用蛋白1抗体
别 名KIAA0552; PRIP1_HUMAN; Proline rich synapse associated protein interacting protein 1; ProSAP-interacting protein 1; ProSAPiP1; ProSAPiP1 protein.
说 明 书0.1ml 0.2ml
研究领域神经生物学 细胞粘附分子 细胞膜蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
ProSAPiP1,含脯氨酸突触相关蛋白相互作用蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量72kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ProSAPiP1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ProSAPiP1,含脯氨酸突触相关蛋白相互作用蛋白1抗体PubMedPubMed
产品介绍background:
The ProSAP family of proteins contain many protein-protein interaction domains and serve as scaffolding mediators within the post-synaptic density (PSD) of excitatory brain synapses. The PSD is an electron-dense structure underneath the post-synaptic plasma membrane of excitatory synapses that anchors and clusters glutamate receptors opposite to the pre-synaptic neurotransmitter release site. Shank proteins contain PDZ modular domains that coordinate the synaptic localization of ion channels, receptors, signaling enzymes, and cell adhesion molecules. The PDZ domain mediates protein-protein interactions via the recognition of a conserved sequence motif at the C-terminus of their target protein(s). ProSAPiP1 (proline rich synapse associated protein interacting protein 1) is a 673 amino acid protein that interacts with the PDZ domain of Shank 3. ProSAPiP1 expression is brain-specific with highest expression within the cerebellum, hippocampus and cerebral cortex.

Subunit:
Interacts (via C-terminus) with SHANK3 (via PDZ domain). Interacts (via coiled coil) with SIPA1L1. Can form homooligomers (By similarity).

Subcellular Location:
Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cytoplasm > cytoskeleton. Detected at synapses, postsynaptic density, synaptic spines and dendrites.

ProSAPiP1,含脯氨酸突触相关蛋白相互作用蛋白1抗体Similarity:
Belongs to the PROSAPIP1 family.

Database links:
UniProtKB/Swiss-Prot: O60299.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti- ProSAPiP1 Polyclonal Antibody, Unconjugated(HZ-11198R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining