Aspartame Assay Kit-相关检验试剂-试剂-生物在线
Aspartame Assay Kit

Aspartame Assay Kit

商家询价

产品名称： Aspartame Assay Kit

英文名称： Aspartame Assay Kit

产品编号： K-ASPTM

产品价格： 0

产品产地： 爱尔兰

品牌商标： Megazyme

更新时间： null

使用范围： null

上海新睿生物科技有限公司
  • 联系人 :
  • 地址 : 上海市徐汇区蒲汇塘路11号博大商务楼1307室
  • 邮编 : 200030
  • 所在区域 : 上海
  • 电话 : 138****4547
  • 传真 :
  • 邮箱 : 3bio@163.com
进入展台

是一种简单可靠的方法，用于饮料和食品中甜味剂的特定测量和分析。

适用于手动，自动分析仪和微孔板格式。
 

UV-method for the determination of Aspartame (and breakdown 
products) in foodstuffs, beverages and other materials

Principle:
                       (pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH

                      (dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine

                      (glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate

                                 (L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+

Kit size:                             * 50 assays (manual) / 500 (microplate) 
                                           / 500 (auto-analyser)
* The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 5 min
Detection limit:                 0.57 mg/L
Application examples: 
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition:         Novel method 
Advantages
 
  • Very cost effective 
  • All reagents stable for > 12 months after preparation 
  • Only enzymatic kit available 
  • Measures aspartame and breakdown products (L-aspartate and aspartame acid) 
  • Very specific 
  • Very rapid reaction 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included 
  • Suitable for manual, microplate and auto-analyser formats