Micro BCA Protein Assay Kit

The Thermo Scientific Pierce Micro BCA Protein Assay is a special 3-component version of our popular BCA Reagents, optimized to measure (A562nm) total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA Reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.

Highlights:

Sensitive– accurately detect down to 0.5µg/mL (2µg/mL in microplate format)

High linearity– linear working range for BSA equals 0.5 to 20µg/mL

Colorimetric– measure with a standard spectrophotometer or plate reader (562nm)

Compatible– unaffected by typical concentrations of most ionic and nonionic detergents

Convenient– microplate and cuvette protocols provided with the instructions

Stable– kit stable at room temperature; prepared working reagent stable for to 24 hours

Product Details:

Protocol summary for the Thermo Scientific Pierce Micro BCA Protein Assay .

Standard curves.Typical standard curves for bovine serum albumin (BSA) and bovine gamma globulin (BGG) in the Micro BCA Protein Assay Kit. The kit includes ampules of Albumin Standard.

References:

1. Smith, P.K., et al. (1985). Anal. Biochem. 150(1), 76-85.
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5. Paratcha, G., et al. (2003). Cell 113, 867-879.