骨骼肌慢肌肌钙蛋白I(TNNI1)重组蛋白

YB229Ra012
Recombinant Troponin I Type 1, Slow Skeletal (TNNI1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
骨骼肌慢肌肌钙蛋白I(TNNI1)重组蛋白[ PROPERTIES ]
Residues: Met1~Gln187
Tags: N-terminal His-Tag
Accession: P19237
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
骨骼肌慢肌肌钙蛋白I(TNNI1)重组蛋白pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and preservative.
Predicted isoelectric point: 9.5
Predicted Molecular Mass: 23.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
p骨骼肌慢肌肌钙蛋白I(TNNI1)重组蛋白recipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS
ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA
DAM L RA L LGS KHKV SM D L RA NLK SV KK E D T EKE RP VE VG D WRK NV EAMSG
MEGRKKMFDA AKSPTSQ
[ REFERENCES ]
1. Corin S.J., et al. (1994) J. Biol. Chem. 269:10651-10659.
2. Wade R., et al. (1990) Genomics 7:346-357.
3. Rual J.F., et al. (2005) Nature 437:1173-1178.
4. Westfall M.V., Borton A.R. (2003) J. Biol. Chem. 278:33694-33700.