大肠杆菌宿主残留蛋白(ECP)重组蛋白

YBX655Ge011
Recombinant Escherichia coli Protein (ECP)   
Organism Species: General
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
大肠杆菌宿主残留蛋白(ECP)重组蛋白9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Ala21~Arg162 (Accession # P23827),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Periplasm. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
大肠杆菌宿主残留蛋白(ECP)重组蛋白sarcosyl and preservative.
Predicted isoelectric point: 7.1
Predicted Molecular Mass: 19.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
大肠杆菌宿主残留蛋白(ECP)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AESVQPLEKI APYPQAEKGM
KRQVIQLTPQ EDESTLKVEL LIGQTLEVDC NLHRLGGKLE NKTLEGWGYD YYVFDKVSSP
VSTMMACPDG KKEKKFVTAY LGDAGMLRYN SKLPIVVYTP DNVDVKYRVW KAEEKIDNAV
VR
[ REFERENCES ]
1. McGrath M.E., et al. (1991) J. Biol. Chem. 266:6620-6625. 2. Lee H.R., et al. (1991) FEBS Lett. 287:53-56. 3. Hayashi K., et al. (2006) Mol. Syst. Biol. 2:E1-E5. 4. Link A.J., et al. (1997) Electrophoresis 18:1259-1313.