DraIII
产品名称： DraIII
英文名称： DraIII
产品编号： HZ600420
产品价格： 0
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： null
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749
- 传真 : 021-60345367
- 邮箱 : www.shzbio.net
DraIII
产品描述
使用说明
高浓度may result in星的酶活性。to obtain 100% activity，BSA should be added to the反应混合到最后1×100μg／ml浓度的BSA。do not use for long孵化。
技术规格
Unit One Unit of the definition酶水解is required to the amount of DNA 1μgλ1小时37°C in at总量在50μl反应。
网站cacnnn↑gtg recognition
带源大肠杆菌基因检测应变，that the III from耐radiophilus博士
我们assayedλDNA
优化的缓冲区（10毫米的2K的Tris-HCl（pH 7.6 at°C）；200毫米10毫米氯化镁；氯化钾；BSA DTT + 1毫米。）
37°C温度的优化
10毫米的存储条件的Tris-HCl（pH 7.5）；300 mM NaCl 0.1毫米1毫米；EDTA；BSA DTT；200μg／ml；50%的甘油。商店在零下20摄氏度。
ligations after 10倍70% of the overdigestion with酶DNA碎片重新ligated and can be。in the Presence of PEG ligation 10%是最好的。
非特异性水解后没有检测到nonspecific of activity was with孵化1μg of DNA酶10 16 hours of UA听说是八周，在37°C。
甲基化效应不灵敏的测试
热灭活的灭活酶是由孵化在150度为20分钟。
campatible adei。
使用说明
High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.?
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Recognition sites | CACNNN↑GTG |
| Source | E.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus |
| Assayed on | Lambda DNA |
| Optimal buffer | 2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) + BSA |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligations | After 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut. In the presence of 10%PEG ligation is better. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Campatible Ends | AdeI |