生物芯片 蛋白质芯片NEXTERION® Slide P广州芯奥光电科技有限公司

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产品名称: 生物芯片 蛋白质芯片NEXTERION® Slide P
英文名称: NEXTERION® Slide P 3-D polymer
产品型号: 蛋白质芯片003
产品规格: 75.6*25.0mm
产品产地: 德国
品牌商标: SCHOTT NEXTERION®
价    格: 150元
更新时间: 2019/3/19 9:57:28
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使用范围:仅限科研使用,不能应用于临床
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广州芯奥光电科技有限公司
地址:
萝岗开发区科学城南云一路2号历康实业大楼3层
邮编:
510663
所在区域:
广东·广州
电话:
020-82510371
传真:
020-82510371
联系人:
曾庆达
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产品详细描述

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Three-dimensional polymer coating 三维薄膜涂层

 

NEXTERION® Slide P

 

SCHOTT specifically developed NEXTERION® Slide P as a dedicated slide surface for printing low density antibody microarrays. NEXTERION® Slide P produces excellent signal-to-background ratios, and exceptionally wide dynamic ranges compared to conventional “two-dimensional” coatings through a unique combination of low non-specific binding characteristics, and high probe loading capacity. The permeable, polymer coating has a large immobilization capacity, and helps to preserve and protect the antibody structure, thus ensuring high specificity and affinity. The robust coating matrix is fully compatible with commercial microarray printers and scanners. A simple printing and processing protocol is available making NEXTERION® Slide P easy to use.

 
 
Overview
 

Type of

coating

Immobilization

method

Typical

probes

Ordering information

NEXTERION®

product

Barcode

option

Item

number

Slides per

pack

3-D polymer

surface

Amine reactive

chemistry 

Covalent binding

AntibodiesSlide PLaser116790425

 

Key product features
• Optimised substrate for printing low density antibody microarrays
• High probe loading capacity
• Extremely low non-specific binding characteristics
• Superior performance for analysis of serum samples
• High probe loading capacity
• Compatible with all common microarray printers and scanners

 

Typical applications
• Validation of serum protein profiles
• Target / biomarker identification
• Proteome expression profiling
• Target / biomarker validation
• Protein expression profiling
• Oncoproteomic profiling
• Cytokine quantification
• Profiling disease states
• Sandwich assays

 

Suitable probe types
• Polyclonal antibodies
• Monoclonal antibodies
• Recombinant antibodies
• Recombinant antibody fragments (scFab, scFv, etc.)

 

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The coating on the SCHOTT NEXTERION® Slide P is a three-dimensional, hydrophilic polymer activated with N-Hydroxysuccinimide (NHS) esters to provide covalent immobilisation of amine groups. All NEXTERION® microarray slides are manufactured from a high quality, low-fluorescence glass coated with low-fluorescence coatings. However the non-specific binding of assay components, especially from serum samples, still remains an important contributor to the off feature background for many microarray applications. For most types of slide coatings the post-print processing protocol involves a method of adsorptive blocking to reduce non-specific binding; however these procedures are difficult to perform in a consistent manner. 


The NEXTERION® Slide P coating has been engineered to exhibit an extremely low intrinsic non-specific background without the need for blocking. This was achieved by using a special polymer that is extremely resistant to non-specific binding. During in-house tests run by SCHOTT, NEXTERION® Slide P had the lowest background signal of any microarray slide coating ever tested. The polymer coating has a three-dimensional structure; with NHS-ester reactive groups attached to the hydrophilic polymer layer. The surface-exposed amino-groups on the antibodies react immediately and irreversibly with the NHS-ester groups forming covalent bonds. The polymer coating maintains the immobilised antibodies in a quasi-liquid environment that maintains their functionality and specificity. The polymer, combined with the end-point attachment, orients the immobilized bio-molecules away from the glass facilitating the interactions of the attached antibodies with the target antigens in the assay sample.

 

Product details

Highly reproducible coating

NEXTERION® Slide P is fabricated using a proprietary deposition process developed by SCHOTT to produce a uniform and reproducible polymer coating on one side of a high quality borosilicate glass slide. All slides are individually examined for physical defects and the absence of particle contamination before and after coating. The coating is applied in a tightly controlled, Class 100 clean room facility, resulting in coated slides with highly uniform surface properties and low auto-fluorescence. 

Excellent spot morphology and signal-to-background ratios

NEXTERION® Slide P provides excellent spot morphologies and reproducible spot sizes over a wide range of probe concentrations.
 
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生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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