MONO S 5/50 GL-蛋白纯化-试剂-生物在线
上海基星生物科技有限公司
MONO S 5/50 GL

MONO S 5/50 GL

商家询价

产品名称: MONO S 5/50 GL

英文名称: MONO S 5/50 GL

产品编号: 17-5168-01

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
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17-5168-01 MONO S 5/50 GL
17-5169-01 MONO S 10/100 GL

 

17-5179-01 MONO Q 4.6/100 PE
17-5180-01 MONO S 4.6/100 PE

Technical Information


MonoBeads™ are prepacked in Tricorn™, HR, and PC column formats. The three Tricorn™ (5/50 GL, 10/100 GL, and 4.6/100 PE) columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads™ columns (see page 511).

 

Tricorn™ columns are ideal for the polishing step when high purity is required. HR 16/10 columns, on the other hand, have higher loading capacity than Tricorn™ columns. PC 1.6/5 columns are ideal for micropurification of subnanogram to microgram amounts of sample on ÄKTA Systems or other high-performance LC systems.

 

Customer feedback on Mono Q HR 5/5.

 

TECHNICAL SPECIFICATIONS
MonoBeads™
Ion exchanger type  Mono Q™  Strong anion 
  Mono S™  Strong cation 
Ionic capacity  Mono Q™  0.27-0.37 mmol Cl-/ml 
  Mono S™  0.12-0.15 mmol H+/ml 
pH stability    2-12 (working), 1-14 (short term) 
Chemical stability    Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid. 
Medium    Monodisperse polystyrene/divinyl benzene particles 
Particle size    10 μm 
Storage    20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) 
Storage temperature  4°C to 30°C 
MonoBeads Columns*
Bed volume  PC 1.6/5  0.1 ml 
  5/50 GL  1 ml 
  4.6/100 PE  1.7 ml 
  10/100 GL  8 ml 
  HR 16/10  20 ml 
Max. pressure  PC 1.6/5  50 bar (725 psi, 5 MPa) 
  5/50 GL, 10/100 GL,
 4.6/100 PE 
40 bar (580 psi, 4 MPa) 
  HR 16/10  30 bar (435 psi, 3 MPa) 
Recommended flow rate  PC 1.6/5  0.01-0.4 ml/min 
  5/50 GL, 4.6/100 PE  0.5-3.0 ml/min 
  10/100 GL  2.0-6.0 ml/min 
  HR 16/10  < 10 ml/min 
* Columns are not suitable for use with ÄKTAprime™ plus system.

 

 

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Tricorn high-performance columns are prepacked with MonoBeads ion exchange media for high-resolution purification and high capacity.

 

Click here to view this image in high resolution
The polishing step on Mono Q 5/50 GL shows different phosphoforms of the purified kinase and very high purity

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165400