17-5170-01
            
MONO P 5/50 GL
        
        

            
17-5171-01
            
MONO P 5/200 GL
        
    


Mono P™ Columns
Technical Information


Mono P™ is based on the same matrix as Mono Q™ and Mono S™. The 10-μm MonoBeads particles are substituted with tertiary and quaternary amines and the small bead size contributes significantly to the high resolution that can be achieved.
 
Reference
 

    
Bhikhabhai, R. et al., Purification, characterization and crystallization of recombinant HIV-1 reverse transcriptase. J. Chromatogr. 604, 157 (1992).

 


    

        

            
TECHNICAL SPECIFICATIONS
        
        

            
Ion exchanger type 
            
Mixed quaternary and tertiary amines 
        
        

            
Charged group 
            
Mixed weak anion 
        
        

            
Ionic capacity 
            
0.15-0.21 mmol Cl-/ml 
        
        

            
Practical loading capacity 
            
5-10 mg/ml medium 
        
        

            
Column dimensions 
            
 
        
        

            
  HR 5/5 
            
5×50 mm 
        
        

            
  HR 5/20 
            
5×200 mm 
        
        

            
Particle size 
            
10 μm 
        
        

            
Matrix 
            
Monodisperse polystyrene/ divinyl benzene 
        
        

            
pH stability 
            
2-12 (long term), 2-14 (short term) 
        
        

            
Chemical stability 
            
Stable in all common buffers except high concentration oxidizing and reducing agents, DMSO, DMF, formic acid, and some detergents. 
        
        

            
Bed volume (bed dimensions) 
            
 
        
        

            
Mono P 5/50 GL 
            
1 ml (5mm × 50mm) 
        
        

            
Mono P 5/200 GL 
            
4 ml (5mm × 200mm) 
        
        

            
Max. pressure 
            
4 MPa (40 bar, 580 psi) 
        
        

            
Recommended flow rate 
            
0.5-1.5 ml/min 
        
        

            
Storage 
            
20% ethanol 
        
        

            
Storage temperature 
            
4°C to 30°C 
        
    

 
 

Characterization of recombinant HIV-1 reverse transcriptase by chromatofocusing on Mono P™. Reproduced with the kind permission of the authors and publisher (1).
 
 

 

The longer, Mono P™ (5 × 200 mm) column gives higher resolution while the shorter, Mono P™ (5 × 50 mm) column gives faster separation. 
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165528