Recombinant Human β-NGF
 
Catalog Number:
AF-450-01
Synonyms:
beta-Nerve Growth Factor
Description:
β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity. Manufactured using all non-animal reagents.
AA Sequence:
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR
Source:
E.coli
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
Assay 1:Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Assay 2:Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
 
 
 
 
Cross Reactivity:
Chicken, Hamster, Mouse, Rat
Research Interests:
Angiogenesis/ Cardiovascular
Chemotaxis
Diabetes / Weight Regulation
Inflammation
Neurobiology
Stem Cells & Differentiation
Wound Healing
 
 
 
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.