RPN2124
            
ECL PLUS WESTERN BLOTTING REAG
        
        

            
RPN2125
            
ECL BLOCKING AGENT
        
        

            
PA43009
            
ECL PLEX G-A-M IGG, CY3, 150UG
        
        

            
PA43010
            
ECL PLEX G-A-M IGG, CY3, 600UG
        
        

            
PA45009
            
ECL PLEX G-A-M IGG, CY5, 150UG
        
        

            
PA45010
            
ECL PLEX G-A-M IGG, CY5, 600UG
        
        

            
PA45011
            
ECL PLEX G-A-R IGG, CY5, 150UG
        
        

            
PA45012
            
ECL PLEX G-A-R IGG, CY5, 600UG
        
        

            
RPN998
            
ECL PLEX PK:CY3,CY5,HYBOND-ECL
        
        

            
RPN999
            
ECL PLEX PK:CY3,CY5,HYBOND-LFP
        
        

            
RPN418
            
ECL PRIME BLOCKING REAGENT
        
        

            
RPN1236
            
ANTI-GST HRP CONJUGATE - 75 UL
        
        

            
25-8006-98
            
ECLBLOCKING AGENT MODULE
        
        

            
28-9011-06
            
ECL Plex GaR IgG Cy3 150ug
        
        

            
28-9011-07
            
ECL Plex GaR IgG Cy3 600ug
        
        

            
RPN2232
            
ECL Prime Western Blotting Det
        
        

            
RPN2232SK
            
ECL Prime Western Blotting Det
        
        

            
RPN850E
            
ECL PLEX FL RAINBOWS 120 UL
        
        

            
RPN851E
            
ECL PLEX FL RAINBOWS 500 UL
        
    



Amersham ECL Prime Western Blotting Detection Reagent

    
High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
    
Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
    
Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm.
    
Compatible with Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers.
    
Optimized for use with Amersham Hybond-P (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.

 
Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.
 
Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

Amersham ECL Prime Western Blotting Detection Reagent — Technical Specifications


    

        

            
Application
            
Sensitive Quantitative or Confirmatory Western Blotting
        
        

            
Recommended Membrane
            
Hybond-P or Hybond ECL
        
        

            
Color
            
Detection reagent 1: Colorless

            Detection reagent 2: Colorless
        
        

            
Storage Conditions
            
2 - 8°C
        
        

            
Sensitivity
            
~1 pg
        
        

            
Detection
            
CCD/Hyperfilm