GenMute™ siRNA转染试剂(免费试用)济南思科生物科技有限公司

性能参数

产品名称: GenMute™ siRNA转染试剂(免费试用)
英文名称: GenMute™ siRNA Transfection Reagent
产品货号: SL100568
产品规格: 0.1毫升、1.0毫升
产品产地: 美国SignaGen
产品商标: SignaGen
价    格: 1980元
保存与运输: 4°C冷藏 常温运输
现货状态: 现货
产品资料: 实验方法技术资料
更新时间: 2019/7/13 13:49:00
浏览人数:13215
诚信指数:164点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

供应商联系卡

济南思科生物科技有限公司
地址:
济南市经六路689号绿地·玫瑰坊A座734号
邮编:
250021
电话:
0531-88807138 85912162
传真:
0531-85912162
联系人:
李先生
所在区域:
山东·济南
邮件:
公司展台:

产品详细描述

 


  产品简介:

GenMute™ siRNA试剂是一种新型的可生物降解的非脂质体的siRNA / miRNA和DNA的载体工具。运用我们专有的PH依赖构象变化技术,这款可生物降解的转染试剂在它的侧链上添加了预筛选出来的疏水集团,使得GenMute™试剂成为市场上最万能的最强大的基因载体工具。GenMute™试剂,已被证实能有效的,可重复性转染单siRN**段和miRNA模拟物或共转染DNA/siRN**段或DNA/miRN**段到各种哺乳动物细胞中。

产品规格:

GenMute™转染试剂,1.0ml,足够在24孔板中转染5.0 pmoles的siRNA或miRNA模拟物达到2000个反应。
- GenMute™ 转染试剂缓冲液(5X),
PGenMute™ 试剂配合使用以获得转染效率最大化,8.0ml(5X)浓缩液就可以制成40ml的工作液体。

应用:
对各种哺乳动物细胞的单siRNA 转染或者 DNA/siRNA 共转染。
对各种哺乳动物细胞的单 miRNA, miRNA模拟物 或者 miRNA抑制剂的转染或者 DNA/miRNA 或DNA/miRNA模拟物的共转染。

储存条件:
40C储存。若储存合适,产品的稳定性能保持12个月以上。

特点:
在只有 1.0 nM siRNA / miRNA模拟物的低浓度中,也能达到非常好的沉默效应。
在细胞内,能将非特异性和非靶目标的影响降到最低。
单管反应。
运用于广泛的哺乳动物细胞系。
细胞毒性低。
物美价廉,非常实惠。

Comparisons of Silencing Efficacy of GenMute™ siRNA & DNA Transfection Reagent with Brand Name Products

 

Knockdown efficacy comparison of GenMute™ Transfection Reagent (upper panel) vs. Dharmafect™ 4 siRNA Transfection Reagent (middle panel) and Lipofectamine™ 2000 (lower panel) on HEK293 cells.siRNA targeting renilla luciferase at different final concentrations ranging from 0.5 to 20 nM was co-transfected with renilla luciferase gene (0.5 µg of pRL-CMV DNA per well) by the above three transfection reagents per manufacturers protocols into HEK293 cells growing on a 24-well plate. Renilla luciferase activity was determined 24h after post co-transfection with renilla luciferase determination system (Promega). The luminescence was measured from 5.0 µl of lysate during 10s integration with a luminometer (Beckman Coulter LD 400). Luciferase activity was expressed as light units integrated over 10s (RLU) and normalized per mg of cell protein by using the BCA assay. The errors bars represent standard deviation derived from triplicate experiments. Luciferase-silencing efficiency was calculated relative to untreated cells. While GenMute™ and Dharmafect™ 4 reagents delivered significant gene silencing from 1.0 nM of renilla luciferase siRNA, lipofectamine™ 2000 gave good knockdown only after 30 nM (data not shown).


Excellent silencing of endogenously expressed KIF11 (also known as Eg5) in HEK293 cells with 0.5 µl of GenMu
te™ reagent and 5.0 pmol Eg5 siRNA per well of 24-well plate. KIF11 (also known as Eg5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. GenMute™ reagent effectively delivers Eg5 siRNA (final 10 nM, right panel) to HEK293 cells at only 0.5 µl per well of 24-well plate vs. negative control (final 10 nM. left panel). Compared with negative control (left panel), phenotype of "rounded-up" 293 cells were visualized 24 hours post transfection (right panel) with a Nikon microscope. 


GenMute™ Transfection Reagent knocked down endogenous lamin A/C gene expression in Hela cells. A siRNA targeting lamin A/C gene (right panel) and a sham siRNA (left panel) were introduced into Hela cells (final 1.0 nM) by GenMute™ Transfection Reagent. Lamin A/C gene silencing was monitored 24h post transfection by immunofluorescence. Lamin A/C was probed with a mouse monoclonal lamin A/C specific antibody followed by addition of FITC-conjugated anti-mouse antibodies. Quantitative analysis showed thatlamin siRNA at 1.0 nM delivered by GenMute™ Transfection Reagent knocked down 94% endogenously expressed lamin A/C in Hela cells.


Data Sheet & Protocol 
A Protocol for siRNA Transfection and DNA/siRNA Co-Tranafection 
A Protocol for miRNA Mimics Transfection and DNA/miRNA Co-Transfection 
A Protocol for DNA Transfection to Mammalian Cells 

 

 

公司官网: http://signagen-china.com

 

 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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