80-6483-56
            
2-D QUANT KIT
        
        

            
80-6483-75
            
MINI DIALYSIS KIT,1 KDA,250 UL
        
        

            
80-6483-94
            
MINI DIALYSIS KIT,1 KDA,2 ML
        
        

            
80-6484-13
            
MINI DIALYSIS KIT,8 KDA,250 UL
        
        

            
80-6484-32
            
MINI DIALYSIS KIT,8 KDA,2 ML
        
        

            
80-6484-51
            
2-D CLEAN-UP KIT
        
        

            
80-6484-70
            
SDS-PAGE CLEAN-UP KIT
        
        

            
80-6501-04
            
2-D FRACTIONATION KIT
        
        

            
80-6501-23
            
PROTEASE INHIBITOR MIX
        
    



2-D Clean-Up Kit
The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D results due to high conductivity, high levels of interfering substances or low concentration

    
Improves the quality of 2-D electrophoresis results by removing interfering contaminants
    
Quantitative precipitation separates proteins from detergents, salts, lipids, phenolics and nucleic acids
    
Delivers quantitative yield in about 90 min.

 
2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution
 
Treatment of the sample with 2-D Clean-Up Kit can improve the quality of 2-D electrophoresis results, reducing streaking, background staining, and other artifacts due to interfering contaminants. The kit can make 2-D analysis possible for samples that are otherwise too dirty or too dilute.
 
The 2-D Clean-Up Kit procedure uses precipitant and co-precipitant in combination to quantitatively precipitate proteins. Precipitated proteins are pelleted by centrifugation and the precipitate is further washed to remove nonprotein contaminants. After a second centrifugation, the resultant pellet is resuspended into denaturing sample solution for first-dimension IEF
 
The procedure can be completed in 90 min and does not result in spot gain or loss. The kit contains sufficient reagents to process 50 samples of up to 100 μl each. The procedure can be scaled up for larger volumes or more dilute samples
 
2-D Clean-Up Kit increases labeling efficiency and protein abundance when using 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE)

2-D Clean-Up Kit — Technical Specifications


    

        

            
Complete Packsize
            
50 samples
        
        

            
Storage Conditions
            
Room temperature. The wash buffer should be placed at -20 °C at least 1 h before use and may be 

            stored in a -20 °C freezer
        
        

            
Wetted Part
            
No
        
        

            
Guidance
            
For removal of interfering contaminants and concentration of total protein

            

            The kit contains 

            15 ml Precipitant 

            17.5 ml Coprecipitant

            50 ml Wash buffer

            250μl Wash additive

            Quick reference card

            Instructions