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Crosslinked Agarose Beads

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Description:
Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is used in different other biomolecules that are used in biological and chemical research, for Affinity Chromatography. Examples include Protein A, Protein G, antibodies, antigens and dyes.

Both resin types give the biomolecules increased stability through the covalent bonds of the enzyme or ligand to the agarose, thus facilitating recovery and later re-use. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker. The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.