官方网址：http://www.sinobiological.cn/H1N1-Hemagglutinin-HA2-Protein-g-6320.html
Influenza A H1N1 ( A/California/04/2009 ) HA2 Protein
 
 
 
 
 
 
 
H1N1 ( A/California/04/2009 ) HA2 Protein Price Inquiry ( Available Sizes )
 
 
 
50μg:  询价;
 
200μg:  询价;
 
≥1mg 大包装:  询价
 
 
 
H1N1 ( A/California/04/2009 ) HA2蛋白产品信息
 
 
 
 
别名:
 
 
HA2
 
 
 
 
表达方式:
 
 
A DNA sequence encoding the Influenza A virus ( A/California/04/2009 (H1N1) ) ( ACP41105.1 ) HA2 subunit ( Gly 345 - Gln 529 ) was fused with the Fc region of mouse IgG1 at the N-terminus
 
 
 
 
种属:
 
Influenza A Virus H1N1
 
 
 
表达宿主: 
 
Human Cells
 
 
 
 
H1N1 ( A/California/04/2009 ) HA2蛋白质控指标
 
 
 
 
纯度:
 
> 95 % as determined by SDS-PAGE
 
SDS-PAGE:
 
 
H1N1 HA2 protein
 
 
 
 
内毒素:
 
< 1.0 EU per μg of the protein as determined by the LAL method
 
 
 
稳定性:
 
Samples are stable for up to twelve months from date of receipt at -70℃
 
 
 
预测N端:
 
Asp
 
 
 
分子量:
 
 
The recombinant Influenza A virus ( A/California/04/2009 (H1N1) ) HA2 / mFc is a disulfide-linked homodimeric protein. The reduced monomer comprises 418 amino acids with the predicted molecular mass of 47.6 kDa. As a result of glycosylation, it migrates as an approximately 55-60 kDa band in SDS-PAGE under reducing conditions
 
 
 
 
缓冲液:
 
Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50mM Tris, pH 7.5 
 
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
 
Please contact us for any concerns or special requirements.
 
 
 
 
 
 
H1N1 ( A/California/04/2009 ) HA2蛋白使用指南
 
 
 
 
储存方法:
 
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
 
 
 
 
 
Reconstitution:
 
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 
 
 
 
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A/California/04/2009
 
 
 
H1N1 ( A/California/04/2009 ) HA2蛋白背景综述
 
 
Hemagglutinin ( HA ) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence. H1N1 is a subtype of influenza virus A and the most common cause of influenza in humans.
 
 
参考文献
 
 
 
Günther-Ausborn, S. et al., 2000, J. Virol. 74: 2714-2720.
 
Hoffman, LR. et al., 1997, J. Virol. 71: 8808-8820.
 
Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-16715.
 
Bonnafous, P. et al., 2000, J. Biol. Chem. 275 : 6160-6166.