17-1184-01
             
RESOURCE ETH 1 ML   *1
         
         
             
17-1185-01
             
RESOURCE ISO 1 ML    * 1
         
         
             
17-1186-01
             
RESOURCE PHE 1 ML   * 1
         
         
             
17-1187-01
             
RESOURCE HIC TEST KIT   1ml*3
         
     
 

          
         
             
17-5186-01
             
SOURCE 15PHE 4.6/100 PE
         
     
 

SOURCE™ Media/RESOURCE™ and Tricorn Columns for HIC
Technical Information

  
 SOURCE™ 15ETH, SOURCE 15ISO, SOURCE 15PHE in media packs and prepacked Tricorn™ and RESOURCE™ columns.
 
 
     
         
             
TECHNICAL SPECIFICATIONS
         
         
             
SOURCE™ Lab Packs
         
         
             
Binding capacity 
             
>   25 mg/ml 
         
         
             
Matrix 
             
Monodisperse polystyrene cross-linked with divinyl benzene 
         
         
             
Particle size 
             
15 μm 
         
         
             
pH stability 
             
2-12 (long term), 1-14 (short term) 
         
         
             
Chemical stability 
             
Stable in common buffers, chaotropic 
             agents, detergents, and polar 
             organic solvents. 
         
         
             
Storage 
             
20% ethanol 
         
         
             
Storage temperature 
             
4°C to 30°C 
         
         
             
RESOURCE™ Prepacked columns
         
         
             
Bed volume 
             
1 ml 
         
         
             
Bed dimensions 
             
6.4 × 30 mm 
         
         
             
Max. pressure 
             
15 bar (1.5 MPa, 217 psi) 
         
         
             
Max. flow rate* 
             
9.6 ml/min 
         
         
             
SOURCE 4.6/100 PE columns (Tricorn™)†
         
         
             
Bed volume 
             
1.7 ml 
         
         
             
Bed dimensions 
             
4.6 × 100 mm 
         
         
             
Max. pressure 
             
40 bar (4 MPa, 580 psi) 
         
         
             
Max. flow rate* 
             
5 ml/min 
         
         
             
* H2O at 25°C.
             † Column not suitable for use with ÄKTAprime™ plus system.             
 
         
     
 

 
 
 The figure on the right shows four runs in an optimization series of eight runs to find the optimal running conditions for the purification of protein tyrosine phosphatase on SOURCE 15PHE 4.6/100 PE (Tricorn™). In the optimization series, the concentration of ammonium sulfate, gradient length, pH, flow rate and additives were varied. The activity was measured by p-nitrophenyl phosphate (pNPP) activity assay at 405 nm. In Run 8, the increase in concentration of ammonium sulfate from 1.5 M to 2.0 M and the increase of the length of the gradient resulted in a better separation.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=10335