SP SEPHAROSE FAST FLOW
产品名称： SP SEPHAROSE FAST FLOW
英文名称： SP SEPHAROSE FAST FLOW
产品编号： 17-0729-01
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
上海基星生物科技有限公司
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983
- 传真 : 021-50801884
- 邮箱 : 342786402@qq.com
| 17-0719-01 | CM SEPHAROSE FAST FLOW 500ML |
| 17-0719-10 | CM SEPHAROSE FAST FLOW, 25 ML |
| 17-0729-01 | SP SEPHAROSE FAST FLOW, 300 ML |
| 17-0729-10 | SP SEPHAROSE FAST FLOW, 25 ML |
| 17-0790-01 | OCTYL-SEPHAROSE CL-4B 200 ML |
| 17-0810-01 | PHENYL-SEPHAROSE CL-4B 200 M |
| 17-0944-01 | SOURCE 15S, 50 ML |
| 17-0944-10 | SOURCE 15S, 10 ML |
| 17-0946-02 | OCTYL SEPHAROSE 4 FAST FLOW 20 |
| 17-0946-10 | OCTYL SEPHAROSE FAST FLOW, 25 |
| 17-0947-01 | SOURCE 15Q, 50 ML |
| 17-0947-20 | SOURCE 15Q, 10 ML |
| 17-0965-05 | PHENYL SEPHAROSE 6 FF LS 200 M |
| 17-0965-10 | PHENYL SEPHAROSE 6 FF / LS, 25 |
| 17-0973-05 | PHENYL SEPHAROSE 6FF (HS) 200 |
| 17-0973-10 | PHENYL SEPHAROSE 6 FF /HS, 25 |
| 17-0978-02 | BUTYL-S SEPHAROSE 6 FF, 200 ML |
| 17-0978-10 | BUTYL-S SEPHAROSE 6 FF, 25ML |
| 17-0980-01 | BUTYL SEPHAROSE 4 FAST FLOW 20 |
| 17-0980-10 | BUTYL SEPHAROSE FAST FLOW, 25 |
| 17-1014-01 | Q SEPHAROSE HIGH PERF. 75 ML |
| 17-1082-01 | PHENYL SEPHAROSE HIGH PERF. 75 |
| 17-1087-01 | SP SEPHAROSE HIGH PERF. 75 ML |
| 17-1273-01 | SOURCE 30S, 50 ML |
| 17-1275-01 | SOURCE 30Q, 50 ML |
| 17-1287-01 | ANX SEPH 4 FF HIGH SUB 500ML |
| 17-1287-10 | ANX SEPHAROSE FF HIGHSUB 25 ML |
| 17-5072-01 | Q SEPHAROSE XL, 300 ML |
| 17-5073-01 | SP SEPHAROSE XL, 300 ML |
| 17-5316-02 | Capto Q 100 ml |
| 17-5316-10 | CAPTO Q, 25 ML |
| 17-5317-02 | Capto MMC 100ml |
| 17-5317-10 | CAPTO MMC, 25ML |
IEX Sepharose™ Fast Flow
- For preparative purifications, even when purifying crude samples.
- Available with strong (Q and SP) and weak (DEAE, CM, and ANX) anion and cation exchangers, in bulk quantities and in prepacked HiTrap™ and HiPrep™ columns, as well as HiTrap™ IEX Selection Kit.
- For separation of proteins of all pI values with Q and SP having a high dynamic capacity for proteins over the entire pH range.
- ANX has a different selectivity compared to DEAE, and is applicable for separation of high molecular weight proteins.
- Easy scale-up from HiTrap™ scale to laboratory scale to process scale.
IEX Sepharose™ Fast Flow
Technical Information
| TECHNICAL SPECIFICATIONS | |
| Ion exchanger type | |
| Q Sepharose™ FF | Quaternary ammonium strong anion |
| DEAE Sepharose™ FF | Diethylaminoethyl weak anion |
| ANX Sepharose™ 4 FF | Diethylaminopropyl weak anion |
| SP Sepharose™ FF | Sulfopropyl strong cation |
| CM Sepharose™ FF | Carboxymethyl weak cation |
| Ionic capacity | |
| Q Sepharose™ FF | 0.18-0.25 mmol (Cl-)/ml |
| DEAE Sepharose™ FF | 0.11-0.16 mmol (Cl-)/ml |
| ANX Sepharose™ 4 FF | 0.13-0.18 mmol (Cl-)/ml |
| SP Sepharose™ FF | 0.18-0.25 mmol (H+)/ml |
| CM Sepharose™ FF | 0.09-0.13 mmol (H+)/ml |
| Dynamic capacity | |
| Q Sepharose™ FF | 120 mg HSA/ml medium |
| DEAE Sepharose™ FF | 110 mg HSA/ml medium |
| ANX Sepharose™ 4 FF | 5 mg thyroglobulin/ml medium |
| SP Sepharose™ FF | 70 mg RNase/ml medium |
| CM Sepharose™ FF | 50 mg RNase/ml medium |
| Pressure/flow spec. | |
| Q Sepharose™ FF | 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| DEAE Sepharose™ FF | 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| ANX Sepharose™ 4 FF | min 200 cm/h, 100 kPa, XK50/60 column, bed height 25 cm |
| SP Sepharose™ FF | 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| CM Sepharose™ FF | 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| Average particle size | 90 μm (45-165 μm) |
| Matrix | |
| Sepharose™ FF | Highly cross-linked agarose, 6% |
| Sepharose™ 4 FF | Highly cross-linked agarose, 4% |
| pH stability | |
| Q Sepharose™ FF | 1-14 (short term), 2-12 (long term) |
| DEAE Sepharose™ FF | 1-14 (short term), 2-13 (long term) |
| ANX Sepharose™ 4 FF | 2-14 (short term), 3-13 (long term) |
| SP Sepharose™ FF | 3-14 (short term), 4-13 (long term) |
| CM Sepharose™ FF | 2-14 (short term), 4-13 (long term) |
| Chemical stability |
Stable in all common aqueous buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*. |
| Storage | 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
| *1 M NaOH and acetic acid should be used only for cleaning purposes.
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Recombinant human superoxide dismutase produced by Saccharomyces cerevisiae is purified directly from clarified cell homogenate on Q Sepharose™ Fast Flow after desalting and buffer exchange on Sephadex™ G-25. A 2.6 × 5 ml ion exchange column handles 390 ml of sample in 45 min.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=8477