Q SEPHAROSE HIGH PERF-蛋白纯化-试剂-生物在线
Q SEPHAROSE HIGH PERF

Q SEPHAROSE HIGH PERF

商家询价

产品名称: Q SEPHAROSE HIGH PERF

英文名称: Q SEPHAROSE HIGH PERF

产品编号: 17-1014-01

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
  • 联系人 :
  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
  • 邮编 : 201900
  • 所在区域 : 上海
  • 电话 : 138****5983
  • 传真 : 021-50801884
  • 邮箱 : 342786402@qq.com

17-1014-01 Q SEPHAROSE HIGH PERF. 75 ML
17-1082-01 PHENYL SEPHAROSE HIGH PERF. 75ML
17-1087-01 SP SEPHAROSE HIGH PERF. 75 ML
17-1273-01 SOURCE 30S, 50 ML
17-1275-01 SOURCE 30Q, 50 ML
17-1287-01 ANX SEPH 4 FF HIGH SUB 500ML
17-1287-10 ANX SEPHAROSE FF HIGHSUB 25 ML
17-5072-01 Q SEPHAROSE XL, 300 ML
17-5073-01 SP SEPHAROSE XL, 300 ML
17-5316-02 Capto Q 100 ml
17-5316-10 CAPTO Q, 25 ML
17-5317-02 Capto MMC 100ml
17-5317-10 CAPTO MMC, 25ML
17-5432-01 Butyl Sepharose HP, 25 ml
17-5432-02 Butyl Sepharose HP, 200ml
17-5437-01 Q SEPHAROSE XL VIRUS LIC 300ML
17-5437-10 Q SEPHAROSE XL VIRUS LIC 25 ML
17-5441-01 CAPTO S, 100 ML
17-5441-10 CAPTO S, 25 ML
17-5443-01 CAPTO DEAE, 100 ML
17-5443-10 CAPTO DEAE, 25 ML
17-5444-10 CAPTO adhere 25 ML

HiLoad™ IEX HP Columns and IEX Sepharose™ High Performance Lab Packs

  • High-performance preparative separations where high efficiency and high resolution are desired.
  • Highly cross-linked agarose matrix provides excellent chemical and physical stability.
  • Available as strong anion exchangers (Q Sepharose HP) and strong cation exchangers (SP Sepharose HP), analogous to Mono Q™ and S, and Q and SP Sepharose™ Fast Flow.
  • Easy to use. For optimal performance and reproducibility, Sepharose™ High Performance is also available in prepacked HiTrap™ columns.

HiLoad™ IEX HP Columns and IEX Sepharose™ High Performance Lab Packs

Technical Information


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Sepharose™ High Performance ion exchangers provide highly efficient, high-resolution preparative scale separations.

 

TECHNICAL SPECIFICATIONS
Ion exchanger type   
Q Sepharose™ HP  Quaternary ammonium strong anion exchanger 
SP Sepharose™ HP  Sulfopropyl strong cation exchanger 
Charged groups   
  SP Sepharose™ HP  - SO3- 
  Q Sepharose™ HP  - N+(CH3)3 
Ionic capacity   
Q Sepharose™ HP  0.14-0.20 mmol Cl-/ml 
SP Sepharose™ HP  0.15-0.20 mmol H+/ml 
Dynamic capacity   
Q Sepharose™ HP  70 mg BSA/ml medium 
SP Sepharose™ HP  55 mg ribonuclease/ml medium 
Rec. flow velocity  30-150 cm/h 
Max. pressure over the packed bed in a HiLoad column during operation  3 bar (42 psi, 0.3 MPa) 
HiLoad™ column hardware pressure limit  5 bar (73 psi, 0.5 MPa) 
Average particle size  34 μm 
Exclusion limit (Mr » 4 ×106 globular protein 
Matrix  cross-linked agarose, 6% 
pH stability   
Q Sepharose™ HP  2-12 (working and long term),
1-14 (short term) 
SP Sepharose™ HP  4-13 (working and long term),
3-14 (short term) 
Chemical stability  Stable in all commonly used buffers:  8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid* 
Storage  20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) 
Storage temperature  4°C to 30°C 
Prepacked HiLoad™ Columns
Bed volume   
HiLoad™ 16/10  20 ml  
HiLoad™ 26/10  53 ml 
Bed dimensions   
HiLoad™ 16/10  16 mm × 100 mm 
HiLoad™ 26/10  26 mm × 100 mm 
Efficiency  > 12 000 m-1 
* 1 M NaOH and 1 M acetic acid should only be used for cleaning.
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