PREPACKED GLUTATHION SEPHAROSE
产品名称： PREPACKED GLUTATHION SEPHAROSE
英文名称： PREPACKED GLUTATHION SEPHAROSE
产品编号： 17-0757-01
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
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| 17-0757-01 | PREPACKED GLUTATHION SEPHAROSE |
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| 27-4810-01 | PEZZ 18 EXPRESION VECTOR |
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| 28-4055-00 | GST MULTITRAP 4B |
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| 28-9523-61 | GST Buffer Kit |
Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification
- Fast and simple purification of GST*-tagged proteins.
- GSTrap™ 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ 4B.
- GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTAdesign.
Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification
Technical Information
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GSTrap 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose 4B for fast purification of GST-tagged proteins and scale-up.
GSTrap™ 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
For an introduction to Glutathione S-transferase (GST) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System.
<bold>3</bold> Eluted pool GSTrap 4B<bold>4</bold> Eluted pool GSTrap 4B (diluted 1:50)<bold>5</bold> Peak 1, HiLoad 16/60 Superdex 200<UNspace/>pg<bold>6</bold> Peak 2, HiLoad 16/60 Superdex 200<UNspace/>pg))
| Columns: | Capture GSTrap 4B 1 ml Polishing HiLoad 16/60 Superdex 200 pg |
| Sample: | Cell extract from E. coli expressing GST-hippocalcin (Mr 45 000) after lysis with CelLytic Express |
| AC binding/wash buffer: | 10 mM sodium phosphate, 140 mM, pH 7.4 |
| AC elution buffer: | 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0 |
| GF buffer: | 50 mM phosphate buffer, 150 mM NaCl, pH 7.2 |
| System: | ÄKTAprime plus |
<bold>3</bold> Eluted pool GSTrap 4B<bold>4</bold> Eluted pool GSTrap 4B (diluted 1:50)<bold>5</bold> Peak 1, HiLoad 16/60 Superdex 200<UNspace/>pg<bold>6</bold> Peak 2, HiLoad 16/60 Superdex 200<UNspace/>pg))
<bold>3</bold> Eluted pool GSTrap 4B<bold>4</bold> Eluted pool GSTrap 4B (diluted 1:50)<bold>5</bold> Peak 1, HiLoad 16/60 Superdex 200<UNspace/>pg<bold>6</bold> Peak 2, HiLoad 16/60 Superdex 200<UNspace/>pg))
1 Low molecular weight markers
2 Start material (diluted 1:20)
3 Eluted pool GSTrap 4B
4 Eluted pool GSTrap 4B (diluted 1:50)
5 Peak 1, HiLoad 16/60 Superdex 200 pg
6 Peak 2, HiLoad 16/60 Superdex 200 pg
| TECHNICAL SPECIFICATIONS | |
| Ligand | Glutathione |
| Ligand coupling method | Epoxy activation |
| Dynamic binding capacity* | > 5 mg horse liver GST/ml medium |
| Matrix | Agarose, 4% |
| Average particle size | 90 μm |
| pH stability | 4-13 |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| HiTrap prepacked columns | |
| Column volume | 1 ml and 5 ml |
| Column dimensions | 0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml) |
| Recommended flow rate† | 0.2-1 ml/min (1 ml); 0.5-5 ml/min (5 ml) |
| Max. pressure† | 3 bar (0.3 MPa) |
| * Binding of GST to glutathione is flow-rate dependent and lower flow rates often iincrease the binding capacity. This is an important consideration, especially during sample loading and elution. † H2O at 25°C.
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