HIS MULTITRAP HP
产品名称： HIS MULTITRAP HP
英文名称： HIS MULTITRAP HP
产品编号： 28-4009-89
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983
- 传真 : 021-50801884
- 邮箱 : 342786402@qq.com
| 28-9546-56 | PGEX-1LT ECOR I/BAP |
| 11-0033-99 | HIS GRAVITRAP |
| 11-0034-00 | HIS BUFFER KIT |
| 27-4710-01 | ANTI-HIS ANTIBODY |
| 28-4009-89 | HIS MULTITRAP HP |
| 28-4009-90 | HIS MULTITRAP FF |
| 28-4013-53 | HIS SPINTRAP |
| 28-4039-43 | COLLECTION PLATE |
| 28-9321-71 | His SpinTrap Kit |
| 27-1583-01 | LIGHT PRIMER MIX |
| 27-1586-01 | HEAVY PRIMER MIX |
| 27-1588-01 | LINKER-PRIMER MIX |
| 27-1589-01 | RS PRIMER MIX |
| 27-9420-01 | ANTI-M13 MONOCLONAL ANTIBODY |
| 27-9421-01 | HRP/ANTI-M13 MONOCLNL CONJUG. |
His MultiTrap 96-Well Filter Plates - High-throughput Screening
- Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* proteins.
- Increased convenience and consistency with prepacked 96-well plates.
- No filtration needed¾load unclarified sample directly, increase reproducibility, and save time.
- Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
- Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns.
- High chemical stability and high binding capacity¾up to 1 mg of histidine-tagged protein per well
His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose™ 6 Fast Flow (His MultiTrap™ FF) or Ni Sepharose™ High Performance (His MultiTrap™ HP). A protocol for His MultiTrap is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.
Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target proteins. His MultiTrap™ FF and His MultiTrap HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
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His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for expression screening and small-scale purification of histidine-tagged proteins.
<sub>6</sub>-NURR1 purified with 96 different buffer conditions on His MultiTrap<TM/> FF. Arrows indicate the position of the target protein. The results show that the highest amount of purified protein was recovered in the buffer at pH 8.5 (25<UNspace/>mM Tris, 100<UNspace/>mM NaCl and 10% glycerol, pH<UNspace/>8.5). This work was performed by R. Steele and B. L. Grasberger at Johnson & Johnson Pharmaceutical Research & Development LCC, USA. Published with permission of the authors.))
SDS-PAGE of (His)6-NURR1 purified with 96 different buffer conditions on His MultiTrap™ FF. Arrows indicate the position of the target protein. The results show that the highest amount of purified protein was recovered in the buffer at pH 8.5 (25 mM Tris, 100 mM NaCl and 10% glycerol, pH 8.5). This work was performed by R. Steele and B. L. Grasberger at Johnson & Johnson Pharmaceutical Research & Development LCC, USA. Published with permission of the authors.
For the full range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.
His MultiTrap 96-Well Filter Plates - High-throughput Screening
Technical Information
| TECHNICAL SPECIFICATIONS | |
| Multiwell format | 96 well |
| Well volume | 800 μl |
| Medium volume | 50 μl |
| His MultiTrap FF His MultiTrap HP |
Ni Sepharose 6 Fast Flow Ni Sepharose High Performance |
| Max. sample loading volume | 600 μl |
| Binding capacity* | |
| His MultiTrap FF His MultiTrap HP |
Up to 800 μg histidine-tagged protein/well Up to 1 mg histidine-tagged protein/well |
| Reproducibility between wells | ± 10% in yield |
| pH stability† | 2-14 (short term), 3-12 (long term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Binding capacity is protein-to-protein dependent. † Ni2+-stripped medium.
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