28-9546-56
            
PGEX-1LT ECOR I/BAP
        
        

            
11-0033-99
            
HIS GRAVITRAP
        
        

            
11-0034-00
            
HIS BUFFER KIT
        
        

            
27-4710-01
            
ANTI-HIS ANTIBODY
        
        

            
28-4009-89
            
HIS MULTITRAP HP
        
        

            
28-4009-90
            
HIS MULTITRAP FF
        
        

            
28-4013-53
            
HIS SPINTRAP
        
        

            
28-4039-43
            
COLLECTION PLATE
        
        

            
28-9321-71
            
His SpinTrap Kit
        
        

            
27-1583-01
            
LIGHT PRIMER MIX
        
        

            
27-1586-01
            
HEAVY PRIMER MIX
        
        

            
27-1588-01
            
LINKER-PRIMER MIX
        
        

            
27-1589-01
            
RS PRIMER MIX
        
        

            
27-9420-01
            
ANTI-M13 MONOCLONAL ANTIBODY
        
        

            
27-9421-01
            
HRP/ANTI-M13 MONOCLNL CONJUG.
        
    


His SpinTrap/His SpinTrap Kit - Minipreps

    
For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
    
Ni Sepharose™ High Performance in prepacked spin columns.
    
Yields up to 750 μg pure protein per column.
    
Allows direct purification of both clarified and unclarified cell lysates

His SpinTrap/His SpinTrap Kit - Minipreps
Technical Information


* For licensing information, see Legal Info.
 
 

His SpinTrap is a single-use spin column for purification of histidine-tagged proteins. For optimal convenience, use His SpinTrap Kit, which contains columns and buffers.
 
His SpinTrap columns
 
His SpinTrap™ is designed for efficient, small-scale purification of histidine-tagged proteins directly from clarified or unclarified cell lysates. The columns are also used for screening of large numbers of small-scale bacterial lysates, as well as optimization of purification conditions.
 
Purification of unclarified samples on His SpinTrap™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
 
His SpinTrap columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap together with buffers prepared using His Buffer Kit, see His Buffer Kit - Convenient, Pre-made Buffers.
 
The columns are prepacked with Ni Sepharose High Performance (see Ni Sepharose™ High Performance - High-performance Purification), which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose High Performance medium is negligible.
 
His SpinTrap Kit
 
The benefits of His SpinTrap and His Buffer Kit are combined in His SpinTrap Kit, which contains one pack of His SpinTrap with 50 columns and one pack of His Buffer Kit. For more information about His Buffer Kit, see His Buffer Kit - Convenient, Pre-made Buffers
 
For the full range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.
 
 

SDS-PAGE (ExcelGel SDS Gradient 8-18) of unclarified and clarified E. coli lysate containing GFP-(His)6 purified using His SpinTrap. Similar purity and recovery was observed from both the unclarified and clarified sample (lanes 4 and 5).
 
 

Purifying histidine-tagged proteins with His SpinTrap is a simple four-stage procedure that can be performed in 10 min using a microcentrifuge:

(1) After placing the column in a 2 ml microcentrifuge tube, equilibrate by adding binding buffer and centrifuge

(2) Add sample, centrifuge

(3) Wash with binding buffer, centrifuge

(4) Elute the target protein with elution by centrifugation
 


    

        

            
TECHNICAL SPECIFICATIONS
        
        

            
Medium 
            
Ni Sepharose High Performance 
        
        

            
Average particle size 
            
34 μm 
        
        

            
Max. sample loading volume 
            
600 μl 
        
        

            
Bed volume 
            
100 μl 
        
        

            
Protein binding capacity* 
            
Approx. 750 μg histidine-tagged protein/column 
        
        

            
Compatibility during use 
            
Stable in all commonly used buffers, reducing agents, denaturants and detergents, see Introduction to Tagged Protein Purification for more information. 
        
        

            
Storage 
            
0.15% Kathon CG 
        
        

            
Storage temperature 
            
4°C to 30°C 
        
        

            
Column material 
            
Polypropylene barrel and polyethylene frits 
        
        

            
* Binding capacity is protein-to-protein dependent
        
    


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