人脂肪细胞--内脏-细胞株/菌种-试剂-生物在线
人脂肪细胞--内脏

人脂肪细胞--内脏

商家询价

产品名称: 人脂肪细胞--内脏

英文名称: HPA-v

产品编号: 7210

产品价格: 0

产品产地: 美国

品牌商标: sciencell

更新时间: 2024-03-27T09:05:42

使用范围: null

上海中乔新舟生物科技有限公司
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  • 邮箱 : wwwfudan@163.com;hufangqiong@zqxzbio.com


上海复蒙基因生物科技有限公司是美国Sciencell公司中国一级代理:

       

       美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售8年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。
 

请登录Sciencell公司官方网站(www.sciencell-sh.comwww.sciencellonline.com)以确保购买正规公司产品。   

 

                
人脂肪细胞--内脏说明:
货号:7210

 

 

脂肪细胞在能量储存和代谢过程中有着重要的作用。脂肪细胞分化是一个发展的过程,它对代谢稳态和营养信号很重要。它由复杂的过程控制,如基因表达和信号转导。前脂肪细胞存在于成人脂肪组织中,能根据能量的平衡增殖和分化成熟的脂肪细胞。前体细胞的增殖和分化能够增加脂肪组织的体积。在体外培养中,不同的组织来源前脂肪细胞的油脂含量,成脂转录因子表达和TNFalpha诱导的凋亡均有不同。同时研究表明它还与脂肪分化以及许多生理病理过程密切相关,如脂肪代谢、能量平衡、肥胖、糖尿病、高血脂和乳癌。

        <7210>人脂肪细胞-内脏提取于内脏脂肪组织,<7200>人脂肪细胞-皮下提取于人皮下脂肪组织,第一代冻存,每管含有细胞数>1×106 cells/ml,此细胞通过CD44, CD90免疫荧光染色和脂肪染色验证,经测试不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌。

推荐培养基:(PAM, Cat. No. 7211)

Description

 

Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction [1]. Preadipocytes are present throughout adult life in adipose tissue and can proliferate and differentiate into mature adipocytes according to the energy balance [2]. The proliferation and differentiation of these preadipocytes contribute to increases in adipose tissue mass. In vitro study indicates that different tissue-derived preadipocytes exhibit differently in lipid accumulation, adipogenic transcription factor expression, and TNF?-induced apoptosis [3]. It has also been demonstrated that there is a close relationship between adipocyte differentiation and many physiological and pathological processes including fat metabolism, energy balance, obesity, diabetes, hyperlipidemia and breast cancer.

HPA-v from ScienCell Research Laboratories are isolated from human visceral fat tissue. HPA-v are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPA-v are characterized by immunofluorescent method with antibodies to CD44, CD90 and lipid staining after differentiation. HPA-v are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPA-v are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.


Recommended Medium

 

It is recommended to use Preadipocyte Medium (PAM, Cat. No. 7211) for the culturing of HPA-v in vitro. Preadipocyte Differentiation Medium (PADM, Catalog No. 7221) can be use for in vitro differentiation of preadipocytes into mature adipocytes, and follows by Adipocyte Medium (AdM, Catalog No. 7201) which maintains mature adipocytes after differentiation.


Product Use

 

HPA-v are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.


Storage

 

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.


Shipping

 

Dry ice.


Reference

 

[1] Tominaga, K., Johmura, Y., Nishizuka, M., Imagawa, M. (2004) Fad24, a mammalian homolog of Noc3p, is a positive regulator in adipocyte differentiation. J Cell Sci. 117(Pt 25):6217-26.
[2] Reue, K., Glueck, S. B. (2001) Accumulating evidence for differences during preadipocyte development: Focus on "Differential gene expression in white and brown preadipocytes". Physiol Genomics. 7(1):1-2.
[3] Tchkonia, T. et al. (2005) Abundance of Two Human Preadipocyte Subtypes with Distinct Capacities for Replication, Adipogenesis, and Apoptosis Varies among Fat Depots. Am J Physiol Endocrinol Metab. 288(1):E267-77.

 
       

17.脂肪细胞系统
    7210 人脂肪细胞-内脏(HPA-v(1×106 )
    7220 人脂肪细胞-皮下(HPA-s(1×106 )
    如需要其他产品资料请发邮件至wwwfudan@163.com索取

        复蒙基因|FMGBio(www.fmgbio.com/   www.sciencell-sh.com/   )成立于2005年, 是专业从事牛血清生产、销售高端的原代细胞类科研产品。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。近几年的新药研发成功率在逐年下降,其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物,其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本,而且将极大地提高人类的健康质量。

        复蒙基因|FMGBio现有的产品:美国Sciencell(原代细胞、原代细胞专用培养基、无血清培养基)、3D Bioteck(三维细胞培养支架)、年产1000万毫升的内蒙古牛血清生产基地等。
       复蒙基因|FMGBioPI团队已经发展到专职PI 20人,联席PI 312人,其中大多数拥有海外背景。截止到20093月,以PI或联席PI为第一作者发表SCI 论文1682 篇(总IF 值为5721.82,其中影响因子IF>5.0 的论文216 篇,IF>10.0论文32 篇)。

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