GST MULTITRAP 4B-蛋白修饰-试剂-生物在线
GST MULTITRAP 4B

GST MULTITRAP 4B

商家询价

产品名称: GST MULTITRAP 4B

英文名称: GST MULTITRAP 4B

产品编号: 28-4055-00

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
  • 联系人 :
  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
  • 邮编 : 201900
  • 所在区域 : 上海
  • 电话 : 138****5983
  • 传真 : 021-50801884
  • 邮箱 : 342786402@qq.com

28-4055-00 GST MULTITRAP 4B
28-4055-01 GST MULTITRAP FF
28-9523-59 GST SpinTrap
28-9523-60 GST GraviTrap
28-9523-61 GST Buffer Kit
28-9545-49 PGEX-4T-1
28-9545-50 PGEX-4T-2
28-9545-52 PGEX-4T-3
28-9545-53 PGEX-5X-1
28-9545-54 PGEX-5X-2
28-9545-55 PGEX-5X-3
28-9546-46 PGEX-2TK
28-9546-48 PGEX-6P-1 VECTOR
28-9546-50 PGEX-6P-2 VECTOR
28-9546-51 PGEX-6P-3 VECTOR
28-9546-53 PGEX-2T 25 UG WI/DI
28-9546-54 PGEX-3X 25 UG WI/DI
28-9546-56 PGEX-1LT ECOR I/BAP
11-0033-99 HIS GRAVITRAP
11-0034-00 HIS BUFFER KIT
27-4710-01 ANTI-HIS ANTIBODY
28-4009-89 HIS MULTITRAP HP
28-4009-90 HIS MULTITRAP FF
28-4013-53 HIS SPINTRAP
28-4039-43 COLLECTION PLATE
28-9321-71 His SpinTrap Kit
27-1583-01 LIGHT PRIMER MIX
27-1586-01 HEAVY PRIMER MIX
27-1588-01 LINKER-PRIMER MIX
27-1589-01 RS PRIMER MIX
27-9420-01 ANTI-M13 MONOCLONAL ANTIBODY
27-9421-01 HRP/ANTI-M13 MONOCLNL CONJUG.

GST MultiTrap 4B

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

  • 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* proteins.
  • Highly reproducible well-to well and plate-to-plate results.
  • High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
  • Wells are prepacked with Glutathione SepharoseTM media for convenience.
  • Easy and predictable scale-up using prepacked GSTrapTM FF, GSTPrepTM FF 16/10, and GSTrapTM 4B columns.

 

GST MultiTrapTM FF and GST MultiTrapTM 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

 

Purification of up to 0.5 mg of GST-tagged proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrapTM filter plates. Direct purification of GST-tagged proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

 

GST MultiTrapTM FF is prepacked with Glutathione SepharoseTM 4 fast flow, while GST MultiTrapTM 4B is prepacked with Glutathione SepharoseTM 4B.

GST MultiTrap 4B — Technical Specifications

Complete Packsize 4 x 96-well filter plates
Storage Conditions 4 - 30°C, 20% Ethanol
Centrifugation Force Recommended 100 x g-500 x g
Centrifugation Force Max. 700 x g
   
Media  
Ligand Glutathione
Average Particle Size 90 μm
Matrix Agarose, 4%
Particle Size 45 µm-165 µm
Binding Capacity/ml Chromatography Medium > 25 mg horse liver GST/ml medium1)
pH stability Working Range 4-13
pH stability Cleaning 4-13
Ligand Coupling Method Epoxy activation
Storage Conditions 4 to 30°C, 20% Ethanol
Chemical Stability All commonly used aqueous buffers
1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.  
   
Column  
Complete Packsize 4 x 96-well filter plates
Number of Wells 96
Sample Volume 600 μl/well
Reproducibility Between Wells ± 10% in yield
Material Polypropylene (PP) and polyethylene (PE).
Dimensions 127.8 x 85.5 x 30.6 mm

Licensing

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