GST SpinTrap
产品名称： GST SpinTrap
英文名称： GST SpinTrap
产品编号： 28-9523-59
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983
- 传真 : 021-50801884
- 邮箱 : 342786402@qq.com
| 28-4055-00 | GST MULTITRAP 4B |
| 28-4055-01 | GST MULTITRAP FF |
| 28-9523-59 | GST SpinTrap |
| 28-9523-60 | GST GraviTrap |
| 28-9523-61 | GST Buffer Kit |
| 28-9545-49 | PGEX-4T-1 |
| 28-9545-50 | PGEX-4T-2 |
| 28-9545-52 | PGEX-4T-3 |
| 28-9545-53 | PGEX-5X-1 |
| 28-9545-54 | PGEX-5X-2 |
| 28-9545-55 | PGEX-5X-3 |
| 28-9546-46 | PGEX-2TK |
| 28-9546-48 | PGEX-6P-1 VECTOR |
| 28-9546-50 | PGEX-6P-2 VECTOR |
| 28-9546-51 | PGEX-6P-3 VECTOR |
| 28-9546-53 | PGEX-2T 25 UG WI/DI |
| 28-9546-54 | PGEX-3X 25 UG WI/DI |
| 28-9546-56 | PGEX-1LT ECOR I/BAP |
| 11-0033-99 | HIS GRAVITRAP |
| 11-0034-00 | HIS BUFFER KIT |
| 27-4710-01 | ANTI-HIS ANTIBODY |
| 28-4009-89 | HIS MULTITRAP HP |
| 28-4009-90 | HIS MULTITRAP FF |
| 28-4013-53 | HIS SPINTRAP |
| 28-4039-43 | COLLECTION PLATE |
| 28-9321-71 | His SpinTrap Kit |
| 27-1583-01 | LIGHT PRIMER MIX |
| 27-1586-01 | HEAVY PRIMER MIX |
| 27-1588-01 | LINKER-PRIMER MIX |
| 27-1589-01 | RS PRIMER MIX |
| 27-9420-01 | ANTI-M13 MONOCLONAL ANTIBODY |
| 27-9421-01 | HRP/ANTI-M13 MONOCLNL CONJUG. |
详情请查询以下网址：http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/zh/GELifeSciences-us/28952359
GST SpinTrap
GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates
- Preserves protein antigenicity and function.
- Yields up to 500 μg of pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
- Allows direct purification of both clarified and unclarified cell lysates.
GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.
GST SpinTrap columns are designed for use in a microcentrifuge. Each column contains 50 μl of Glutathione Sepharose 4B, enough for purifying up to 500 μg of recombinant GST and each package contains 50 columns. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged proteins.
GST SpinTrap — Technical Specifications
| Complete Packsize | 50 x 50 µl |
| Bed Volume | 50 µl |
| Binding Capacity/Column | Up to 500 µg recombinant glutathione S-transferase (rGST) |
| Storage Conditions | 4 to 30°C, PBS and 0,05% Kathon CG/ICP Biocide |
| Media | |
|---|---|
| Ligand | Glutathione |
| Average Particle Size | 90 μm |
| Matrix | Agarose, 4% |
| Particle Size | 45 µm-165 µm |
| Binding Capacity/ml Chromatography Medium | > 25 mg horse liver GST/ml medium1) |
| pH stability Working Range | 4-13 |
| pH stability Cleaning | 4-13 |
| Storage Conditions | 4 to 30°C, 20% Ethanol |
| Chemical Stability | All commonly used aqueous buffers |
| 1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |