GST GraviTrap
产品名称： GST GraviTrap
英文名称： GST GraviTrap
产品编号： 28-9523-60
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983
- 传真 : 021-50801884
- 邮箱 : 342786402@qq.com
| 28-4055-00 | GST MULTITRAP 4B |
| 28-4055-01 | GST MULTITRAP FF |
| 28-9523-59 | GST SpinTrap |
| 28-9523-60 | GST GraviTrap |
| 28-9523-61 | GST Buffer Kit |
| 28-9545-49 | PGEX-4T-1 |
| 28-9545-50 | PGEX-4T-2 |
| 28-9545-52 | PGEX-4T-3 |
| 28-9545-53 | PGEX-5X-1 |
| 28-9545-54 | PGEX-5X-2 |
| 28-9545-55 | PGEX-5X-3 |
| 28-9546-46 | PGEX-2TK |
| 28-9546-48 | PGEX-6P-1 VECTOR |
| 28-9546-50 | PGEX-6P-2 VECTOR |
| 28-9546-51 | PGEX-6P-3 VECTOR |
| 28-9546-53 | PGEX-2T 25 UG WI/DI |
| 28-9546-54 | PGEX-3X 25 UG WI/DI |
| 28-9546-56 | PGEX-1LT ECOR I/BAP |
| 11-0033-99 | HIS GRAVITRAP |
| 11-0034-00 | HIS BUFFER KIT |
| 27-4710-01 | ANTI-HIS ANTIBODY |
| 28-4009-89 | HIS MULTITRAP HP |
| 28-4009-90 | HIS MULTITRAP FF |
| 28-4013-53 | HIS SPINTRAP |
| 28-4039-43 | COLLECTION PLATE |
| 28-9321-71 | His SpinTrap Kit |
| 27-1583-01 | LIGHT PRIMER MIX |
| 27-1586-01 | HEAVY PRIMER MIX |
| 27-1588-01 | LINKER-PRIMER MIX |
| 27-1589-01 | RS PRIMER MIX |
| 27-9420-01 | ANTI-M13 MONOCLONAL ANTIBODY |
| 27-9421-01 | HRP/ANTI-M13 MONOCLNL CONJUG. |
详情请查询以下网址：
http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/zh/GELifeSciences-us/28952360
GST GraviTrap
GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein
- Convenient, prepacked gravity-flow columns.
- Allows direct purification of both clarified and unclarified cell lysates.
- Yields up to 20 mg of pure proteins per column.
- Yields up to 20 mg of pure proteins per column
GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein. GST-tagged proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the proteins.
The binding capacity of prepacked GST GraviTrap columns, which can be greater than 10 mg protein/ml medium, varies with the nature of the tagged protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged proteins.
GST GraviTrap — Technical Specifications
| Complete Packsize | 10 x 2 ml |
| Bed Volume | 2 ml |
| Binding Capacity/Column | Up to 20 mg recombinant glutathione S-transferase (rGST) |
| Storage Conditions | 4 to 30°C, 20% Ethanol |
| Media | |
|---|---|
| Ligand | Glutathione |
| Average Particle Size | 90 μm |
| Matrix | Agarose, 4% |
| Particle Size | 45 µm-165 µm |
| Binding Capacity/ml Chromatography Medium | > 25 mg horse liver GST/ml medium1) |
| pH stability Working Range | 4-13 |
| pH stability Cleaning | 4-13 |
| Storage Conditions | 4 to 30°C, 20% Ethanol |
| Chemical Stability | All commonly used aqueous buffers |
| 1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |
| Column | |
|---|---|
| Column i.d. | 15 mm |
| Chemical Stability | Stable in all commonly used buffer systems |
| Material [Column Hardware] | Polypropylene (PP) |
| Material [Bed Support] | Polyethylene (PE) |